钙调素拮抗剂对长春花碱抗癌作用的影响  

The effect of calmodulin antagonist on the anticancereffect of vinblastine

作　　者：刘树人[1] 张雁 陆汉明[1] 赵雅丽 林仲翔 

机构地区：[1]上海第二医科大学附属新华医院,北京市肿瘤研究所

出　　处：《中华医学杂志》1994年第11期680-682,共3页National Medical Journal of China

摘　　要：为了解钙调素（ＣａＭ）活性与抗肿瘤药物细胞毒性之间的关系，采用ＭＧＣ８０３胃腺癌细胞系研究钙调素拮抗剂（Ｗ－７）对长春花碱抗癌作用的影响。用ＣａＭ－ＰＤＥ活性测定法研究Ｗ－７对ＣａＭ生物活性的影响；用细胞培养法研究Ｗ－７、长春花碱对细胞增殖的影响；用放射性同位素标记脉冲示踪法研究Ｗ－７对胞内长春花碱浓度的影响。实验结果表明，２５μｍｏｌ／ＬＷ－７能增加胞内长春花碱的浓度（Ｐ＜０．仍），降低其半数抑制量（Ｐ＜０．０５），提高其抗癌效应。almodulin exisrs in all eukaryotic cells. It func-tions as the intracellular receptor of Ca, regulatesvarious cellular physiological processes. We studiedthe effect of calmodulin antagonist W-7 on the anti-cancer effects of vinblastine. With the method thatcalmodulin can activate cyclic nucleotide PDE, wefound that W-7 can significantly reduce the calmod-ulin level of MGC803 cell (P<0. 05). W-7 could notonly increase the uptaking and accumulating of-3H-Vinblastine in MGC803 cells (P<0. 05) , but alsodecrease the IC50 of Vinblastine (from 12. 0 ± 0.03nmol to 5. 27±0. 02 nmol) in MGC803 cells. It isindicated that calmodulin antagonist W-7 can enhancethe anticancer effect of vinblastine.

关 键 词：钙调素 钙通道阻滞 长春碱 抗肿瘤药 药理学 

分 类 号：R979.19[医药卫生—药品]