一种新型HLA衍生肽对大鼠脾细胞免疫功能的抑制作用  

Inhibiting effect of HLA-derived peptide on immune function of rat splenocytes

作　　者：易善红[1] 汪泽厚[2] 宋波[3] 叶钢[1] 

机构地区：[1]第三军医大学附属新桥医院泌尿外科,重庆400037 [2]解放军空军总医院泌尿外科,北京100036 [3]第三军医大学附属西南医院全军泌尿中心,重庆400038

出　　处：《中国免疫学杂志》2005年第6期421-423,共3页Chinese Journal of Immunology

基　　金：国家新药基金资助项目(9690105184)

摘　　要：目的:观察一种新型HLA衍生肽(RDP1258)的免疫抑制功能并探讨其机制。方法:人工固相合成一种HLA衍生肽(RDP1258),3H-TdR掺入法检测其在体外对大鼠脾细胞增殖反应的影响,并采用酶化学法观察其对脾细胞血红素氧化酶-1(hemeoxygenase-1,HO-1)活性的影响。结果:RDP1258可显著抑制淋巴细胞转化及MLR的增殖反应;该肽体外降低HO-1活性呈现出剂量依赖性。结论:RDP1258能够显著抑制大鼠脾细胞经丝裂原或同种抗原刺激引起的增殖反应,这种作用可能是通过影响HO-1活性而达到的。Objective: To study the immunosuppression function of a novel HLA-derived pepride, RDP1258,and it' s mechanisms. Methods:A peptide derived from HLA,RDP1258,was chemically synthesized.The effects of the peptide on alloreactive cytotoxic activities of rat splenocytes were observed using 3H-TdR method.The heme oxygenase-1(HO-1) activity was analyzed by the enzyme chemistry method.Results:The results showed that the synthetic HLA-derived peptide can obviusly inhibit the proliferation of rat splenocytes and the peptide inhibited HO-1 activty in a dose-dependment manner in vitro.Conclusion:HO-1 might participate in the RDP1258 inhibiting the proliferation of rat splenocytes induced by mitogen and isoantigen.

关 键 词：HLA衍生肽 混合淋巴细胞反应 血红素氧化酶-1 

分 类 号：R392.11[医药卫生—免疫学]