CpG-ODN活化人NK细胞的初步研究  

Preliminary study on human immune cells activated by CpG-ODN

作　　者：李宁[1] 范学工[1] 陈立章[1] 陈朝晖[2] 刘洪波[1] 

机构地区：[1]中南大学湘雅医院传染科,长沙410008 [2]中南大学湘雅医院医学实验中心,长沙410008

出　　处：《中国免疫学杂志》2005年第6期424-426,共3页Chinese Journal of Immunology

基　　金：本课题受湖南省科技攻关计划项目(04SK3040-3)资助

摘　　要：目的:研究D型CpG-ODN对人免疫细胞的活化效应。方法:CpG-ODN体外刺激人外周血单个核细胞(PBMC),ELISA检测培养液IFN-α及IFN-γ的含量;RT-PCR检测PBMC中TLR9的表达水平;MTT法观察活化的NK对K562的杀伤作用。结果:CpG-ODN有效诱导PBMC分泌IFN-α和IFN-γ,增强活化的NK细胞对K562细胞的杀伤作用,且能显著上调TLR9mRNA的表达。结论:在TLR9的介导下,CpG-ODN能有效激活NK细胞,参与机体免疫调节。Objective:To study immunological stimulation of synthetic oligodeoxynucleotides(ODN) containing unmethylated CpG dinucleotides(CpG-ODN) on human immune cells.Methods:CpG-ODN was co-cultured with peripheral blood mononuclear cells(PBMC) .The IFN-α and IFN-γ in the supernatant were measured by ELISA;the reverse transcription PCR was used to analyze the expression levels of TLR9 mR NA in PBMC;MTT method was used to detect NK-mediated lytic activity of K562 cells.Results:CpG-ODN induced high amounts of IFN-α as well as IFN-γ production;the lysis of K562 mediated by CpG-ODN 2216 stimulating NK cells was increased;the CpG-ODN could up-regulate the expression of TLR9 mRNA in PBMC. Conclusion: CpG-ODN 2216 can activate human immune reaction by increasing the production of IFN-α and IFN-γ,the expression of TLR9 and NK cell lytic activity.

关 键 词：CPG寡脱氧核苷酸 Α干扰素 TOLL样受体9 外周血单个核细胞 

分 类 号：R392.11[医药卫生—免疫学]