抗人TNF-α单克隆抗体对银屑病患者血清TNF-α的中和研究  

The neutralized study of anti-human TNF-α monoclonal antibody on the TNF-α in sera of the psoriatic patients

作　　者：冯峥[1] 王建平[1] 葛萍[1] 李恒进[1] 黎燕[2] 

机构地区：[1]解放军总医院皮肤科,北京100853 [2]军事医学科学院基础医学研究所,北京100850

出　　处：《军医进修学院学报》2005年第3期225-227,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家863计划重大项目资助(2002AA2Z344B)

摘　　要：目的:研究抗人TNF-α单克隆抗体对寻常型银屑病患者血清TNF-α的中和效应。方法:应用双抗体夹心ELISA方法检测正常人及患者血清应用单克隆抗体中和前后TNF-α的浓度改变。应用L929细胞检测正常人和患者血清抗体中和前后的生物学活性变化情况。结果:正常人与银屑病患者血清TNF-α的浓度分别为:(3.146±6.184)pg/ml及(62.723±38.379)pg/ml;其生物学活性分别为:(6.817±5.086)%及(57.498±9.125)%。抗体中和后患者血清TNF-α的浓度为:(0.503±1.178)pg/ml;中和后的生物学活性为:(25.378±14.863)%。结论:寻常型银屑病患者血清中TNF-α的浓度及生物学活性均高于正常对照,而抗人TNF-α单克隆抗体在体外可以很好地中和患者血清中TNFα的浓度及其生物学活性。Objective:To study the neutralization effect of anti-human TNF-α monoclonal antibody on the TNF-α in sera of the psoriatic patients. Methods:We used sandwich ELISA to detect the concentrations of TNF-α in sera of healthy volunteers and patients before and after neutralization with antibody. L929 cells were Used to detect the bioactivities of TNF-α in sera of healthy volunteers and patients before and after neutralization with antibody. Results:The concentrations of TNF-α in sera of healthy volunteers and patients were(3.146±6.184) pg/ml and(62.723±38.379) pg/ml respectively;their bioactivities were(6.817±5.086) % and(57.498±9.125)% respectively. After neutralization with antibody, the concentrations of TNF-α of patients were(0.503±1.178)pg/ml, and the bioactivities of TNF-α of patients were(25.378±14.863)%. Conclusions:We can find that the concentrations and bioactivities of TNF-α in sera of patients were elevated, and anti-human TNF-α monoclonal antibody can well neutralize the concentrations and bioactivities of TNF-α in psoriatic patients’ sera.

关 键 词：肿瘤坏死因子 抗人TNF-α 单克隆抗体 中和试验 银屑病 血清TNF-Α 血清抗体 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]