纳洛酮对急性心肌梗死再灌注后梗死面积和肌酸激酶同工酶活性的影响  

Effect of naloxone on the infarcted size and the activity of MB isoenzyme of creatine kinase after acute myocardial ischemia-reperfusion injury

作　　者：汤晓琴[1] 赵建洪[2] 张正义[3] 宋少莉[4] 张煦[5] 

机构地区：[1]兰州医学院解剖教研室 [2]兰州医学院第二附属医院放射科 [3]兰州医学院第二附属医院急诊中心 [4]兰州医学院第一附属医院核医学中心 [5]兰州医学院病理解剖教研室,甘肃省兰州市730000

出　　处：《中国临床康复》2005年第19期22-23,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:通过制造大鼠急性心肌梗死再灌注损伤模型,探讨纳络酮对大鼠急性心肌梗死再灌注损伤的保护作用。方法:实验于2003-05/10在兰州医学院解剖教研室实验室完成。成年SD大鼠30只随机分为手术对照组、单纯缺血再灌注组、缺血加纳络酮组,每组10只。用戊巴比妥钠(40mg/kg)腹腔注射麻醉大鼠,从根部结扎左冠状动脉前降支制备心肌缺血-再灌注模型。单纯缺血再灌注组在结扎左冠状动脉前降支心肌缺血30min后,剪断结扎线恢复心肌再灌注4.5h。缺血加纳洛酮组在术前10min及结扎后0.5h,4h,静脉注射纳洛酮0.517mg/kg。其余两组注射同体积的生理盐水。处死大鼠后迅速摘取心脏;;经处理后氯化三苯基四氮唑法染色和数码照相计算机图象分析系统计算心肌梗死面积;;测定血清中肌酸激酶同工酶含量。结果:30只大鼠全部进入结果分析。缺血加纳洛酮组与单纯缺血再灌注组比较;;心肌梗死面积明显缩小犤(37.53±5.77)%;;(26.73±3.67)%;;P<0.01犦;;血清中肌酸激酶同工酶活性明显降低犤(210.59±17.87)kat/L;;(153.12±22.16)kat/L;;P<0.01犦。结论:纳洛酮可明显缩小缺血再灌注心梗面积;;降低血清肌酸激酶同工酶含量;;改善心功能。AIM:To explore the protective role of naloxone in rats with acute myocardial ischemia reperfusion injury through establishing the models. METHODS:The experiment was carried out in the laboratory of Staff Room of Hum an Anatomy of Lanzhou Medical College from May to October 2003.Thirty adult SD r ats were randomly divided into three groups:operated control group(n=10),simple ischemia reperfusion group(n=10) and ischemia+ naloxone group(n=10).The rats were anesthetized with intraperitoneal injection of pentobarbital sodium(40 mg/k g),and then they were made into models of ischemia reperfusion by ligating the left anterior descending coronary brand from root.In the simple ischemia repe rfusion group,ligature was cut for recovery of myocardial reperfusion for 4.5 ho urs at 30 minutes after ligation of left anterior descending coronary brand.In t he ischemia+ naloxone group,the rats received venous injection of naloxone(0.51 7 mg/kg) 10 minutes before operation and 30 minutes and 4 hours after ligation r espectively,and rats in the other groups were injected with saline of the same v olume.The rats were then sacrificed and hearts were harvested immediately for de termination of myocardial infarcted size with the triphenyl tetrazolium chloride method and digital photography computer aided video system,and the serum conten t of creatine kinase isoenzyme was detected. RESULTS:All the 30 rats were involved in the analysis of results.Compared the ischemia+ naloxone group with the simple ischemia reperfusion group,the myoc ardial infarcted size obviously became smaller[(37.53± 5.77)% , (26.73± 3.6 7)% ,P< 0.01],serum activity of MB isoenzyme of creatine kinase was significant ly decreased [(210.59± 17.87) kat/L,(153.12 ± 22.16) kat/L,P< 0.01]. CONCLUSION:Naloxone can reduce obvisouly the myocardial infarcted size, decre ase the serum content of MB isoenzyme of creatine kinase and ameliorate cardiac function of patients with ischemia reperfusion.

关 键 词：阿片受体拮抗剂/药理学 心肌再灌注损伤 心肌梗塞 肌酸激酶同工酶类/血液 灌注 局部 

分 类 号：R542.2[医药卫生—心血管疾病]