脊髓后角神经元蛋白激酶C活化与哮喘及地塞米松的关系  

Relationship of the activation of protein kinase C with asthma and dexamethasone in the spinal cord posterior horn neurons

作　　者：谷春雨[1] 曹德寿[2] 刘春霞[3] 方秀斌[2] 

机构地区：[1]中国医科大学附属第二医院神经外科,辽宁省沈阳市110003 [2]中国医科大学神经生物学教研室,辽宁省沈阳市110001 [3]沈阳医学院奉天医院妇科,辽宁省沈阳市110024

出　　处：《中国临床康复》2005年第19期55-57,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(30070330);辽宁省教育厅基金资助项目(20121032)~~

摘　　要：目的:探讨蛋白激酶C在哮喘豚鼠C7～T5段脊髓后角的表达及地塞米松的抑制作用。方法:实验于2003-03/06在中国医科大学神经生物实验室进行,健康白色豚鼠40只,随机分为4组:①生理盐水组8只,腹腔注射生理盐水1mL,10d后雾化吸入生理盐水,隔日1次,共20d。②单纯致敏组8只,腹腔注射100g/L卵蛋白(含等量氢氧化铝凝胶)1mL,10d后雾化吸入生理盐水,隔日1次,共20d。③哮喘组12只,腹腔注射100g/L卵蛋白(含等量氢氧化铝凝胶)1mL,10d后雾化吸入10g/L卵蛋白溶液(不含氢氧化铝凝胶),隔日1次,共20d。④地塞米松组12只,操作同哮喘组,从激发哮喘后第6天起,每天于哮喘后腹腔注射地塞米松犤2mg/(kg·d)犦,1次/d,共5次。利用免疫组织化学和免疫蛋白印迹观察哮喘豚鼠C7～T5段脊髓后角蛋白激酶C(参与支气管平滑肌增殖)蛋白变化。结果:40只豚鼠均进入结果分析。C7～T5段脊髓后角蛋白激酶C的表达变化:生理盐水组与单纯致敏组豚鼠表达基本相近(0.113±0.009,0.116±0.007,t=1.554,P>0.05);;哮喘组豚鼠表达比生理盐水组明显上调(0.176±0.010,t=23.033,P<0.01),地塞米松组表达则明显低于哮喘组(0.128±0.009,t=17.359,P<0.01)。结论:哮喘豚鼠内脏感觉传入部位C7～T5段脊髓后角蛋白激酶C呈过表达,应用地塞米松可抑制蛋白激酶C表达的增加。此结果进一步揭示了哮喘发病过程中有神经免疫机制的参与。AIM:To investigate the expression of protein kinase C in the C7 T5 spinal cord posterior horn and the inhibitory effect of dexamethasone in asthmatic guinea pigs.METHODS:The experiment was carried out in the neurobiological laboratory of China Medical University from March to June 2003.Forty healthy white guinea pigs were randomly divided into 4 groups:① Saline group(n =8):guinea pigs received intraperitoneal injection of 1 mL saline, after 10 days,saline aerosol was used every two days for 20 days.② Simple sensitization group(n=8):guinea pigs were sensitized by intraperitoneal injection of 1 mL ovalbumin(100 g/L,containing aluminum hydroxide gel of the same volume),after 10 days,saline aerosol was used every two days for 20 days.③ Asthma group(n=12):guinea pigs were sensitized by intraperitoneal injection of 1 mL ovalbumin(100 g/L,containing aluminum hydroxide gel of the same volume),after 10 days,ovalbumin aerosol(not containing aluminum hydroxide gel) was used every two days for 20 days.④ Dexamethasone group(n=12):guinea pigs were treated the same as those in the asthma group;From the 6th day after stimulation of asthma,they received daily intraperitoneal injection of dexamethasone(2 mg/kg per day) for five times.The alterations of protein kinase C protein,which participated in the proliferation of bronchial smooth muscle,in C7- T5 spinal cord posterior horn of asthmatic guinea pigs were observed with immunohistochemistry and Western blot.RESULTS:All the 40 guinea pigs were involved in the analysis of results.The alterations of protein kinase C protein in C7- T5 spinal cord posterior horn:The expressions were close between the saline group and simple sensitization group(0.113± 0.009,0.116± 0.007,t=1.554,P >0.05);It was obviously up regulated in the asthma group(0.176± 0.010) as compared with the saline group(t=23.033,P< 0.01);It was markedly lower in the dexamethasone group(0.128± 0.009) than in the asthma group(t =17.359,P< 0.01).CONCLUSION:The protein kinase C over expresses in the C7- T5 spinal

关 键 词：蛋白激酶C 地塞米松 脊髓 哮喘 

分 类 号：R562.2[医药卫生—呼吸系统]