2型糖尿病心血管并发症风险因子与血管紧张素I转换酶等位基因和基因型频率的关联  

Association of the risk factors of cardiovascular complication with the alleles and genotypic frequency of angiotensin I converting enzyme in type 2 diabetes mellitus

作　　者：邢朝斌[1] 

机构地区：[1]华北煤炭医学院生物科学系普通生物学教研室,河北省唐山市063000

出　　处：《中国临床康复》2005年第19期124-125,共2页Chinese Journal of Clinical Rehabilitation

基　　金：河北省科技厅科技攻关项目(022761106)~~

摘　　要：目的:探讨血管紧张素Ⅰ转换酶基因多态性与2型糖尿病心血管并发症风险因子的相关性。方法:实验于2001-02/2002-06在华北煤炭医学院分子生物学实验室完成。对象均为华北煤炭医学院附属医院门诊及住院的2型糖尿病患者,符合1985年世界卫生组织2型糖尿病诊断标准,心肌梗死诊断根据MRI确诊,均知情同意。根据是否合并心肌梗死分为心肌梗死组68例和非心肌梗死组190例。按常规酚/氯仿法提取基因组DNA,血管紧张素Ⅰ转换酶基因扩增产物用20g/L琼脂糖凝胶电泳分析。对不同患者组等位基因(I,D)和基因型频率(DD,DI,II)进行比较。相对风险>1为等位基因和基因型与疾病有肯定关联(风险因子)。结果:两组患者全部进入结果分析。2型糖尿病心肌梗死组DD纯合子频率明显高于非心肌梗死组犤41.2%,33.2%(χ2=0.833,P<0.05)犦,D等位基因频率也较非心肌梗死组显著增高犤64.7%,55.0%(χ2=3.8491,P<0.05)犦。DD基因型及D等位基因的相对风险分别为1.33和1.50。结论:D等位基因和DD基因型是2型糖尿病心肌梗死发生的风险因子。AIM:To investigate the relationship between angiotensin I-converting enzyme gene polymorphism and the risk factors of cardiovascular complications in type 2 diabetes mellitus.METHODS:The experiment was carried out in the Laboratory of Molecular Biology,North China Coal Medical College from February 2001 to June 2002.The subjects were the type 2 diabetic patients,who were treated or hospitalized in the affiliated hospital,they all met the diagnostic criteria of type 2 diabetes mellitus set by WHO in 1985,and the diagnosis of myocardial infarction was according to MRI.All the subjects participated in the study voluntarily.They were divided in to myocardial infarction group(n=68) and non myocardial infarction group(n=190) according to whether the patients were accompanied by myocardial infarction or not.Genome DNA was extracted with the method of routine phenol/chloroform,the product of and angiotensin I converting enzyme gene amplification was analyzed with 20 g/L agarose gel electrophoresis.The alleles(I,D) and genotypic frequency(DD,DI,II) were compared between different patient groups.If the relative risk >1,the allele and genotype had certain association with the disease,and they were the risk factors.RESULTS:All the patients in both groups were involved in the analysis of results. The frequency of DD homozygote was obviously higher in the type 2 diabetic myocardial infarction group than in the non myocardial infarction group(41.2% ,33.2% ,χ 2=0.833,P< 0.05),the frequency of D allele was also significantly increased as compared with the non myocardial infarction group(64.7% ,55.0% ,χ 2=3.849 1,P< 0.05).The relative risks of DD genotype and D allele were 1.33 and 1.50 respectively.CONCLUSION:D allele and DD genotype are the risk factors for the occurrence of type 2 diabetic myocardial infarction.

关 键 词：糖尿病 非胰岛素依赖型 心肌梗塞 肽基二肽酶A 多态现象 

分 类 号：R587.1[医药卫生—内分泌]