高血压左室肥厚发病中细胞间黏附分子1的表达及丹参酮ⅡA的抑制效应(英文)  

Expression of intercellular adhesion molecule-1 in the development of hypertensive left ventricular hypertrophy and inhibitory effect of tanshinoneⅡA

作　　者：占成业[1] 陶秀良[2] 田橙[1] 熊玮[1] 郑智[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,湖北省武汉市430030 [2]华中科技大学同济医学院附属同济医院中西医结合研究所,湖北省武汉市430030

出　　处：《中国临床康复》2005年第19期254-256,共3页Chinese Journal of Clinical Rehabilitation

基　　金：湖北省自然科学基金资助项目(2000J064)~~

摘　　要：背景:细胞间黏附分子1作为炎症反应的可靠标志物在动脉粥样硬化发病中具有重要的作用。近年来研究认为其介导的慢性炎症反应可能参与高血压左室肥厚的发病过程,但也有报道持相反观点。丹参酮ⅡA是丹参的一种脂溶性提取性,动物实验证明其具有抑制高血压左室肥厚发生的作用。目的:探讨细胞间黏附分子1在高血压左室肥厚发生中的作用及丹参酮ⅡA对其表达的影响。设计:随机对照观察。单位:华中科技大学同济医学院附属同济医院。材料:实验于2002-10/2004-01在华中科技大学同济医学院附属同济医院急诊科实验室完成,选用12周龄雄性WKY大鼠10只、自发性高血压SHR大鼠20只,分为对照组(WKY大鼠10只)、高血压组(SHR大鼠10只)、丹参酮ⅡA组(SHR大鼠10只)。方法:丹参酮ⅡA组经尾静脉注射丹参酮ⅡA1.5mg/(kg·d)治疗,其他两组分别注射等量的蒸馏水。12周后断头处死所有大鼠留取心肌标本,应用苏木精-伊红染色、VG染色,免疫组化染色及心肌ED1标记检测心肌巨噬细胞浸润数,心肌细胞间黏附分子1mRNA及蛋白的表达应用反转录-聚合酶链反应及酶链免疫吸附实验等方法。主要观察指标:各组大鼠心肌巨噬细胞浸润数,心肌细胞间黏附分子1mRNA及蛋白表达。结果:20只SHR和10只WKY大鼠被纳入实验,并全部进入结果分析,无脱失值。①与对照组比较,高血压组大鼠肥厚心肌中细胞间黏附分子1的mRNA及蛋白表达显著增加(0.176±0.087,0.537±0.195;0.104±0.011,0.173±0.027,P<0.01或P<0.05),巨噬细胞浸润明显(0.62±0.07,1.85±0.23,P<0.01)。②与高血压组比较,丹参酮ⅡA组大鼠心肌细胞间黏附分子1的mRNA及蛋白表达水平显著下调(0.537±0.195,0.291±0.106;0.173±0.027,0.125±0.014,P<0.01或P<0.05),巨噬细胞浸润数减少(1.85±0.23,1.16±0.17,P<0.05),心肌细胞肥大和间质纤维化程度明显减轻。结论:心肌细胞间黏附分子1的过度表BACKGROUND: As a reliable marker of inflammation,intercellular adhesion molecule-1(ICAM-1) plays an important role in atherogenesis.Recently,it is assumed in researches of recent years that chronic inflammation mediated by ICAM-1 is involved in hypertensive left ventricular hypertrophy probably,but there are still some objections reported.TanshinoneⅡA is a kind of liposoluble extract from danshen(Radix Salviae Mitiorrhizae).It is verified in animal experiment that it can inhibit hypertensive left ventricular hypertrophy.OBJECTIVE: To investigate the relationship between ICAM-1 and hypertensive left ventricular hypertrophy and the effect of tanshinoneⅡ A on expression of ICAM-1.DESIGN: A randomized controlled observation was designed.SETTING:Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology.MATERIALS: The experiment was performed in the Laboratory of Emergency Department of Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from October 2002 to January 2004. Ten male WKY rats(10 WKY rats) of 12 week old and 20 rats with spontaneous hypertension(20 SHR rats) were employed and divided into the control group(10 WKY rats), hypertension group(10 SHR rats) and tanshinone Ⅱ A group(10 SHR rats).METHODS: In Tanshinon II A group,tanshinoneⅡ A 1.5 mg/(kg· d) was injected from caudal vein for treatment and distilled water at the same dose was injected in the other two groups. Twelve weeks later, the rats were sacrificed and myocardium was collected for specimen preparation.Haematoxylin and eosin( HE) staining,VG staining,immunocytochemical staining and myocardial ED1 labeling were applied to determine myocardial macrophage infiltration degree.The reverse transcription polymerase chain reaction(RT PCR) and enzyme linked immunosorbent assay(ELISA) were used to determine the expressions of ICAM 1 mRNA and protein.MAIN OUTCOME MEASURES: Degree of macrophages infiltration and the expressions of ICAM 1 mRNA and protein i

关 键 词：高血压 肥大 左心室 细胞间粘附分子 巨噬细胞 丹参酮 

分 类 号：R544.1[医药卫生—心血管疾病]