大鼠胚胎中脑神经前体细胞培养、移植治疗大鼠PD模型  

Implantation of in vitro Expanded Mesencephalic Progenitor Cells of Rats for Management of Parkinsonian Rats

作　　者：费力 江澄川 冯林音[2] 季耀东 丁忠良[3] 

机构地区：[1]复旦大学附属华山医院神经外科,200040 [2]中国科学院上海生命科学院神经科学研究所,200031 [3]复旦大学上海医学院解剖与组织胚胎学系,200032

出　　处：《中国临床神经科学》2005年第2期193-197,共5页Chinese Journal of Clinical Neurosciences

摘　　要：目的:研究原代培养的大鼠胚胎中脑前体细胞(mesencephalicprogenitorcells ,MPC)作为帕金森病(Parkinson’sdisease ,PD)细胞替代疗法供体的可行性。方法:PD模型大鼠19只,随机分为细胞移植组(n =9)、假移植组(n =5 )和对照组(n =5 ) ;定期测定移植前后各模型鼠阿扑吗啡诱导的旋转行为的变化,免疫组化定性供体细胞在宿主体内存活和分化的情况。结果:术后6和16周,细胞移植组阿扑吗啡诱导旋转的相对频率与术前比较有显著差异;细胞移植组术后16周时的阿扑吗啡诱导旋转的相对频率平均值与对照组对应的相对频率平均值相比也有显著差异;供体细胞能在宿主体内分化为多突起的DA能神经元。结论:原代培养的胚胎MPC可能作为PD患者细胞替代治疗的供体细胞。Aim:To study the feasibility of in vitro expanded mesencephalic progenitor cells(MPC) of rats being used as a new approach in cell replacement therapy of Parkinson's disease.Methods:In vitro expanded MPC of E12 rats was trypsinized after 5 to 7 days in vitro and re-suspended in D-Hank's solution at a density of 120,000 viable cells per microlitre.In cell grafted group ( n =9),four microlitre cell suspension was grafted into the lesioned stritum of hemiparkinsonian rat;in sham transplanted group ( n =5),four microlitre D-Hank's solution was injected in the same way;the other 5 hemiparkinsonian rats served as a control group.The apomorphine-induced rotation test were recorded regularly,the survival and differentiation of grafted cells were evaluated by immunohistochemistry at 6 months post-transplantation.Results:The cell grafted rats exhibited a marked reduction in relative apomorphine-induced rotation rate at 6 and 16 weeks post-transplantation.Compared with the control group,the average relative apomorphine-induced rotation rate in cell grafted group at 16 weeks post-transplantation was decreased significantly too.The grafted cells could differentiated into large,multipolar dopamine(DA) neurons in lesioned stritum and survived more than 6 months after intrastritum transplantation.Conclusion:In vitro expanded MPC of E12 rats could differentiated into DA neurons in the lesioned stritum and had a pronounced effect on apomorphine-induced turning behavior to the hemiparkinsonian rat.It might served as a new donor in cell replacement therapy of Parkinson's disease.

关 键 词：细胞培养 大鼠胚胎 PD模型 移植治疗 脑神经 disease 细胞替代疗法 细胞移植 阿扑吗啡 供体细胞 DA能神经元 细胞替代治疗 原代培养 cells 前体细胞 胚胎中脑 帕金森病 模型大鼠 旋转行为 免疫组化 体内分化 对照组 平均值 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学] R445.1[医药卫生—基础医学]