鲁道夫对盲囊线虫rDNAITS遗传标记的研究  

MOLECULAR CHARACTERIZATION OF CONTRACAECUM RUDOLPHII USING RIBOSOMAL ITS-1AND ITS-2 MARKERS

作　　者：何芳[1] 翁亚彪[1] 林瑞庆[1] 李安兴[2] 朱兴全[1] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]中山大学生命科学学院,广州510275

出　　处：《寄生虫与医学昆虫学报》2005年第2期77-81,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家杰出青年科学基金项目 (3 0 2 2 5 0 3 3 )

摘　　要：本实验通过对鲁道夫对盲囊线虫(Contracaecumrudolphii)rDNA的第一及第二内转录间隔区(ITS- 1及ITS -2 )进行PCR扩增、PCR SSCP分析及序列分析,以明确ITS 1及ITS 2是否可作为C. rudolphii分子分类的遗传标记。结果发现:C .rudolphiirDNAITS序列存在种间差异,并且差异显著,但是种内序列一致,没有差异。本实验证明C rudolphii确为由两个种(C .rudolphiiA和C rudolphiiB)组成的复合种,ITS -1及ITS- 2可作为两个姊妹种的遗传标志。The aim of the present study was to assess whether the first(ITS-1)and second(ITS-2)internal transcribed spacers(ITS)of ribosomal DNA(rDNA)could be used as genetic markers for the identification of members of the Contracaecum rudolphii complex.ITS-1 and ITS-2 were amplified by PCR separately from C rudolphii and the amplicons were subjected to single-strand conformation polymorphism(SSCP)analysis,followed by selective sequencing.While no variation in single-stranded ITS-1 and ITS-2 profiles was detected among samples representing each of the species,SSCP analysis of the ITS-1 and ITS-2 amplicons allowed the unequivocal differentiation of all of the three species of Contraceacum examined.Each of the three species had its unique ITS-1 and ITS-2 sequences.The findings of the study supported that C rudolphii comprises two sibling species,C rudolphii A and C rudolphii B.These results indicated that ITS-1 and ITS-2 sequences provide reliable genetic markers for the specific identification of C rudolphill A,C rudolphill B and C.septentrionale.

关 键 词：鲁道夫对盲囊线虫 RDNA ITS 遗传标记 PCR-SSCP 序列分析 鸬鹚 

分 类 号：R383.1[医药卫生—医学寄生虫学]