石英染尘大鼠早期肺灌洗液细胞中羟脯氨酸等指标变化  

Changes of Cellular Hydroxyl Proline and Some Other Parameters of Rats in Early Bronchoalveolar Lavage Treated with Quartz Dust

作　　者：秦孝发[1] 刘世杰[1] 

机构地区：[1]北京医科大学劳动卫生教研室

出　　处：《中华预防医学杂志》1994年第2期88-90,共3页Chinese Journal of Preventive Medicine

基　　金：WHO资助课题

摘　　要：对不同剂量（５、１０、２０、４０ｍｇ）石英粉尘染尘两周大鼠肺灌洗液细胞中羟脯氨酸等指标变化进行研究。结果表明，各染尘组肺灌洗液中细胞分类、细胞中羟脯氨酸及总脂、上清液中总脂、总蛋白、乳酸脱氢酶、铜蓝蛋白及全肺胶原含量均出现明显变化。进一步分析表明，以肺灌洗液细胞中羟脯氨酸与全肺胶原之间的相关性最好（γ＝０．８３４），说明该指标能较好地反映染尘早期全肺胶原含量的增加。he changes of cellular hydroxyl proline(Hyp) and some other parameters in bronchoalveolarlavage(BAL)induced by different doses of quartz dust(5,10, 20, or 40mg / per rat) were studied.Quartz dust was administered intratracheally and the rats were killed two weeks after quartz administra-tion. The results showed that the differential count of polymorphonuclear leukocytes(PMN)in BAL,the concentrations of Hyp and total lipids in BAL cells,and the levels of total lipids,total protein,lactic dehydrogenase(LDH) and ceruloplasmin in BAL supernatant all changed significantly in quartz treated groups compared with the control group.Further investigation revealed that the cellular Hyp correlated best with total lung collagen(γ=0.834).This indicated that Hyp might be a specific and important parameter reflecting early increase of total lung collagen after quartz dust admimstration.

关 键 词：矽肺 羟脯氨酸 肺泡灌洗液 细胞 

分 类 号：R598.202[医药卫生—内科学]