FK506对GAP_(-43)和Tau蛋白活性影响的实验研究  

Experimental study of the effect of FK506 on GAP_(-43) and Tau protein activity

作　　者：陈泽华[1] 张振伟[1] 帅建中[1] 廖坚文[1] 林冷[1] 庄加川[1] 张家俊[1] 黄汉文[1] 

机构地区：[1]深圳市沙井人民医院手外科,深圳市手外科治疗康复中心,深圳518104

出　　处：《中华手外科杂志》2005年第3期183-185,共3页Chinese Journal of Hand Surgery

基　　金：广东省科技厅科技攻关基金资助项目(2003C33812);深圳市科技局科研基金资助项目(200204179)

摘　　要：目的观察FK506缓释膜片对大鼠神经损伤后生长相关蛋白(Growthassociatedprotein43,GAP43)和Tau蛋白活性的影响。方法将FK506缓释膜片(释放速度为1mg·d,共21d)放置于损伤坐骨神经周围为实验组,损伤坐骨神经周围不放置药物者为对照组。于术后3d,术后1、2、3、4、6和8周共7个时间组,分别取神经缝合口近端和远端的神经段、腰段脊神经节及脊髓腰膨大,用SABC法检测GAP43及Tau蛋白的阳性标记量。结果实验组于术后1～4周已出现GAP43及Tau蛋白表达增强,与对照组相比差异有统计学意义(P<0.01)。结论FK506能增加GAP43及Tau蛋白表达,对周围神经损伤后的神经再生有一定的促进作用。Objective To observe the influence of FK506 on GAP -43 and Tau protein activity after nerve injuries in Wistar rats. Methods Sciatic nerve injury model was established in Wister rats. In the experimental group, FK506 was delivered via a slow-release film that was wrapped around the repair site. In the control group, only nerve repair was done. At 3 days,1, 2, 3, 4, 6 and 8 weeks after the operation,the proximal and distal end of the neurorrhaphy, dorsal root ganglia, and spinal cord were harvested. Expression of GAP-43 and Tau protein were detected by determine the labelled amount using SABC method. Results In the experimental group, the expression of GAP -43 and Tau protein was increased 1 to 4 weeks postoperatively. The difference was statistically significant. Conclusion FK506 can increase the expression of GAP -43 and Tau protein in the peripheral nerve. It thus might promote nerve regeneration following nerve injuries.

关 键 词：GAP-43 TAU蛋白 实验研究 活性影响 FK506缓释膜片 损伤坐骨神经 生长相关蛋白 周围神经损伤 脊髓腰膨大 SABC法 蛋白活性 释放速度 脊神经节 阳性标记 表达增强 蛋白表达 神经再生 实验组 对照组 术后 缝合口 统计学 放置 

分 类 号：R651.3[医药卫生—外科学] R749.16[医药卫生—临床医学]