左心辅助对急性左心衰犬心肌局部AngII受体mRNA表达的实验研究  

The experimental study of left ventricular assist device on local expression of AngII receptors mRNA of left ventricle in acute left heart failure canine model

作　　者：李占清[1] 路万里[1] 陈晨[1] 蒲国华[1] 张宇[1] 陈国生[1] 

机构地区：[1]华北煤炭医学院附属医院心胸外科,唐山063000

出　　处：《中华胸心血管外科杂志》2005年第1期35-37,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：原煤炭部科学基金 (97- 382 );唐山市青年科技明星计划 (962 1 55)资助

摘　　要：目的　用心脏局部AngII受体AT1和AT2mRNA表达评价左室辅助装置 (LVAD)的有效性。方法　 15只健康成年杂种犬 ,随机均分为 3组 :对照组只插管而不造成心衰 ,另两组制做急性左心衰模型 ,其中一组中等流量 (70ml·kg-1·min-1)对辅助组犬进行 3h辅助。于不同时段分别在梗死区左室游离壁钻取全层心肌以RT PCR法测定心脏局部AT1和AT2mRNA的表达。结果　心衰组AT1mRNA在急性左心衰竭后左室局部表达明显降低 (P <0 . 0 1) ,且随缺血时间延长进一步下降 ;AT2mRNA表达升高(P <0 . 0 1) ,且随缺血时间延长进一步上升。LVAD组左心室局部AT1mRNA表达于不同时段均显著高于心衰组水平 (P <0. 0 1) ;AT2mRNA表达均明显低于心衰组水平 (P <0. 0 5 )。结论　LVAD可逆转左室局部AT1mRNA表达下降及AT2mRNA表达上升 ,为临床治疗提供理论基础。同时亦证明了LVAD的有效性。Objective To evaluate the effect of left ventricular assist device(LVAD) on acute left heart failure canine model by the alteration of local AngII receptor(AT1/AT2) mRNA expression in left ventricular myocytes. Methods 15 canines were randomly divided into 3 groups: Control, hert failure(HF) and HF+LVAD groups. Acute left heart failure model was induced by occluding the left anterior descending coronary artery (LAD) or LAD+left circumflex coronary artery (LCX). The LVAD group was treated for 3 hours by pneumatic LVAD, whose output was 70?ml·kg. -1.·min. -1.. 100mg myocardium was taken from the left ventricle of each dog at 1, 2, 3 hours respectively. The expression of the local left ventricle AngII receptor (AT1/AT2) mRNA was tested by reverse transcription-polymerase chain reaction (RT-PCR). Results When compare with the control group, the expression of the AT1 mRNA in ischemic left ventricle decreased significantly in HF group after heart failure; AT2 mRNA in ischemic left ventricle increased significantly in HF group after heart failure. At 1, 2, 3 hours after LVAD, the AT1 mRNA level of the HF+LVAD group was higher than that of the HF group's, the AT2 mRNA level of the LVAD group was lower than that of the HF group's. Conclusion After heart failure, the expression of AT1 mRNA decreased, but it can be reversed by the LVAD. The expression of AT2 mRNA increased, and it can be reversed by the LVAD. The improvement of the AT1 mRNA and AT2 mRNA values in HF+LVAD group shows the improvement of left ventricular function.

关 键 词：MRNA表达 AngⅡ受体 实验研究 左心辅助 犬心肌 RT-PCR法 左室辅助装置 急性左心衰竭 LVAD 心脏局部 不同时段 时间延长 AT2 成年杂种犬 AT1 心衰模型 理论基础 临床治疗 ATI 对照组 游离壁 梗死区 左心室 可逆转 下降 

分 类 号：R654.2[医药卫生—外科学]