烧伤小鼠血清中促造血成分的毛细管电泳分析  

Analyzing the hematopoietic stimulating factors in the burned mice serum by capillary electrophoresis

作　　者：周燕虹[1] 罗成基[1] 郭朝华[1] 孔佩艳[1] 邹仲敏[1] 

机构地区：[1]第三军医大学军事预防医学院全军复合伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2005年第11期1061-1064,共4页Journal of Third Military Medical University

基　　金：全军"九五"重点课题 ( 96L0 4 5)~~

摘　　要：目的　分析烧伤后血清中造血刺激活性成分。方法　毛细管电泳分析烧伤血清及经离子交换层析所得造血活性组分A、B、C。结果　①烧伤血清经毛细管电泳后发现有6个主要变异峰不同于正常血清。②经离子交换分离后得到的具有造血活性的3种中的各亚组分(除3 3 6×10 3外) ,均与全血清毛细管电泳图谱中相应未知的变异峰的变化趋势相吻合。③A、B、C 3种组分经毛细管电泳分析,其主要成分分子量A为10×10 3、B为3 3 6×10 3、83 9×10 3、C为41 2×10 3、48 2×10 3。④10×10 3和48×10 3蛋白与目前已报道的造血活性细胞因子分子量不一致。结论　烧伤后血清中产生了某些促进造血的物质。10×10 3、48×10 3蛋白的分子量与已报道的造血刺激因子的分子量不一致,其性质及功能有待进一步研究。Objective To examine whether abnormal hematopoietic activity components occurs in burned sera and identify their characteristic. Methods Serum samples harvested form burned mice and their fractions from ion-exchange chromatography were separated by capillary electrophoresis (CE) and were their molecular weights investigated. Results There were six abnormal peaks in burned sera different from control. Every subfraction separated from A, B, C fraction by CE appeared at the corresponding site in total serum CE profile and coincided with the abnormal peak respectively, except for 33.6×10 3 protein. The molecular weight detected by CE of fraction A is 10×10 3, fraction B 33.6×10 3 and 83.9×10 3, and C 41.2×10 3 and 48.2×10 3. The 10×10 3 protein in fraction A and 48×10 3 protein in fraction C did not match with any other reported hematopoietic cytokines. Conclusion Some stimulating hematopoietic proteins appeared in burned sera. Further research about 48×10 3 and 10×10 3 protein modulating hematopoiesis after burn injury is necessary.

关 键 词：烧伤 造血调控 毛细管电泳 

分 类 号：R331.1[医药卫生—人体生理学] R446.112[医药卫生—基础医学]