普罗托品对大鼠血管平滑肌收缩反应及细胞内游离钙浓度的影响  

Effect of protopine on contraction and intracellular free calcium concentration of smooth muscle of thoracic aorta in rats

作　　者：李斌[1] 吴芹[1] 孙安盛[1] 石京山[1] 黄燮南[1] 

机构地区：[1]遵义医学院药理学教研室,遵义563003

出　　处：《第三军医大学学报》2005年第11期1097-1099,共3页Journal of Third Military Medical University

基　　金：贵州省省长基金资助项目 [黔科教办 ( 2 0 0 1) 4号 ]~~

摘　　要：目的　探讨普罗托品(protopine ,Pro)对大鼠血管平滑肌细胞内游离钙浓度的影响。方法　采用无Ca2 + 复Ca2 + 的实验法,间接观察Pro对大鼠血管平滑肌细胞内游离钙浓度([Ca2 + ] i)的可能影响,再利用钙荧光指示剂Fura 2 AM负载的培养大鼠胸主动脉平滑肌细胞,直接观察在Pro存在下,对去甲肾上腺素(NA)和高钾所致的[Ca2 + ] i 的升高的影响。结果　在无钙Krebs液中,Pro 10、3 0、10 0 μmol L对NA所致血管条的短暂收缩均有明显的浓度依赖性抑制作用,对复Ca2 + 后NA所诱发的持续收缩也呈剂量依赖性抑制作用。在Fura 2 AM负载血管平滑肌细胞的实验表明,Pro (5 0 μmol L ,10 0 μmol L)对静息时的[Ca2 + ] i 无明显影响;在有外钙存在条件下,Pro 5 0 μmol L能明显降低NA所致[Ca2 + ] i 的升高,对KCl引起的[Ca2 + ] i升高有降低趋势;当Pro的浓度增加到10 0 μmol L时,对NA和KCl引起的[Ca2 + ] i 升高均有明显的抑制作用。结论　Pro可能通过抑制Ca2 + 释放和(或)Ca2 + 内流来降低NA和高钾所致血管平滑肌[Ca2 + ] i 的升高,从而抑制血管平滑肌的收缩反应。Objective To study the effect of protopine (Pro) on the intracellular free calcium ions of the smooth muscular cells of the thoracic aorta in rats. Methods The procedure of calcium absence-calcium addition was designed to observe the changes of the level of calcium ions in the strips of the smooth muscle of the thoracic aorta indirectly. The concentration of calcium ions in the smooth muscle of the thoracic aorta was determined with Fura-2/AM loaded SMCs. The elevation of calcium ions was induced with norepinephrine (NE). The concentration of potassium ions was observed in the presence of Pro. Results Pro significantly inhibited the NE-induced transient contract of the smooth muscle of the thoracic aorta in calcium-free medium and long-lasting contraction after the addition of calcium ions in a concentration-dependent manner. In Fura-2/AM loaded SMCs, Pro (50 μmol/L or 100 μmol/L) exerted no effect on the resting calcium ions but obvious effect on the NE-induced and high potassium level-induced elevation of calcium ions. In the presence of extracellular calcium ions, Pro (50 μmol/L) decreased the NE-induced elevation of calcium ions but showed no effect on potassium-induced elevation of calcium. Pro (100 μmol/L) significantly decreased NE-induced and high potassium level-induced elevation of calcium ions. Conclusion Pro reduces NE-induced or high potassium level-induced elevation of calcium ions in the smooth muscle of the thoracic aorta through its effect on calcium release and/or calcium influx.

关 键 词：普罗托品 细胞内游离钙 大鼠胸主动脉条 血管平滑肌细胞 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R337.2[医药卫生—基础医学]