雪旺细胞与神经元共培养时NGF、CNTF和GDNF mRNA共表达的计算机成像  

Computerized imaging of the co-expression of NGF,CNTF and GDNF mRNA in Schwann cells cocultured with neurons

作　　者：陈菁[1] 任勇[2] 楚燕飞[1] 陈志强[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第六研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042 [2]重庆大学通信工程学院,重庆400030

出　　处：《第三军医大学学报》2005年第12期1240-1243,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目 ("973"项目 ) (G19990 54 2 0 6 ) ;第三军医大学科研基金资助项目 (XG2 0 0 34 8)~~

摘　　要：目的　研究雪旺细胞与背根节神经元共培养条件下多种神经营养因子共表达的计算机数字成像技术。方法　运用多色荧光原位杂交技术和计算机图像处理技术,对相同视野下分别采用绿、红、蓝单色滤光片摄取的NGF(绿)、CNTF(红)和GDNF(蓝)mRNA表达的荧光图像以及明视场细胞图像进行其共表达分布的图像合成。结果　在单个雪旺细胞内同时显示出NGF、CNTF和GDNFmRNA表达所对应绿、红、蓝色区域及其在细胞相同部位同时表达所对应的由绿、红、蓝色混合而衍生的白、黄、紫红和浅蓝色区域。结论　应用多色荧光原位杂交技术和计算机图像处理技术可对雪旺细胞中多种神经营养因子的共表达进行可视化研究。Objective To study the computerized digital imaging of the co-expression of multiple neurotrophic factors in Schwann cells cocultured with neurons from dorsal ganglion. Methods The green, red and blue fluorescent images respectively corresponding to the expression of NGF, CNTF and GDNF mRNA and the image of Schwann cells under bright field were composed into one image which can reveal the co-expression of above 3 factors simultaneously by means of multicolor fluorescent in situ hybridization and computer image processing technique. Results The green, red and blue area corresponding to the expression of NGF, CNTF and GDNF mRNA and the white, yellow, mauve and azury area corresponding to the co-expression of any 2 or 3 factors in the same site resulting from the various combination of red, green and blue color were showed on the level of single schwann cell. Conclusion The visualized study of the co-expressions of multiple neurotrophic factors in Schwann cells is available.

关 键 词：共表达 雪旺细胞 多色荧光原位杂交 计算机成像 

分 类 号：R319[医药卫生—基础医学] R322.8