乌司他丁对严重烫伤大鼠早期心肌损害保护作用  

Protective effects of ulinastatin on myocardial damage following early burn injury

作　　者：谢康[1] 黄跃生[1] 周军利[1] 张家平[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,创伤,烧伤,复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2005年第12期1244-1246,共3页Journal of Third Military Medical University

基　　金：国家杰出青年科学基金资助项目 ( 30 12 50 4 0 ) ;全军"十五"计划指令性课题 ( 0 1L0 6 6 ) ;教育部高等学校骨干教师资助计划项目( 2 0 0 0 ) ;全军首批临床高新技术重大项目 ( 2 0 0 2 )~~

摘　　要：目的　探讨乌司他丁(ulinastatin ,UTI)对烧伤早期“休克心”的防治效果。方法　72只SD大鼠随机等分为单纯烫伤组(B组)和烫伤后乌司他丁治疗组(U组) ,建立3 0 %体表面积Ⅲ度烫伤模型。检测烫伤前及伤后1、3、6、12h和2 4h血浆cTnI和CK MB浓度,观察心肌组织病理学变化。结果　烫伤后3、6、12、2 4h大鼠血浆cTnI、CK MB均显著高于伤前(P <0 0 1) ,U组各时相点cTnI、CK MB均显著低于B组(P <0 0 1或P <0 0 5 )。伤后3h ,光镜下可见心肌纤维肿胀,横纹紊乱,细胞界限不清、消失,胞浆破坏,细胞间质水肿,伤后6～12h病变加重,可见心肌组织点片状溶解灶等。乌司他丁处理组病变显著减轻。结论　严重烧伤早期即发生明显的心肌损害,乌司他丁能够显著减轻严重烧伤时心肌损害的程度,对烧伤后“休克心”的防治具有显著作用和疗效。Objective To investigate the preventive and therapeutic effects of ulinastatin (UTI) on myocardial damage following early burn injury. Methods A total of 72 SD rats were randomly divided into simple burn group (B group) and UTI-treated group (U group) and were subjected to 30% total body surface area Ⅲ scald injury. Plasma cTnI and CK-MB concentrations were measured by enzyme linked immunosorbent assay and histologic changes in myocardium were examined before and at 1, 3, 6, 12 and 24 h after burn. Results Serum cTnI and CK-MB levels at 3, 6, 12, 24 h after burn injury were significantly higher than before burn (P<0.01). Serum cTnI and CK-MB levels in U group were lower than in B group (P<0.01, P<0.05). At 3 h after burn, fiber engorgement, transverse striation derangement, cell boundary unsharpness, cytoplasm destruction, intercellular substance edema were observed. The pathomorphological changes were aggravated at 6 and 12 h after burn injury, and dissolvation, degeneration and necrosis occurred, which were mild as compared with that in B group. Conclusion Severe myocardial damage occurred following early burn injury. Ulinastatin could alleviate myocardial damage.

关 键 词：烧伤 心肌损害 乌司他丁 

分 类 号：R542.2[医药卫生—心血管疾病] R644[医药卫生—内科学]