G-CSF对不同人群CD34^+细胞及T细胞亚群影响的动态变化研究  

Dynamic changes of CD34^+ cell and T lymphocyte subset from different populations after G-CSF treatment

作　　者：王存邦[1] 陈幸华[1] 欧英贤[2] 白海[2] 魏亚明[2] 欧建锋[2] 慕海霞[2] 王晓静[2] 

机构地区：[1]第三军医大学新桥医院血液科,重庆400037 [2]兰州军区总医院全军血液病中心,兰州730050

出　　处：《第三军医大学学报》2005年第12期1269-1271,共3页Journal of Third Military Medical University

摘　　要：目的　探讨应用G CSF动员外周血干细胞时,异体 自体造血干细胞移植供 受者的骨髓及外周血CD3 4+ 细胞和T细胞亚群的动态变化特点及最佳外周血干细胞采集时间。方法　对12例健康供者和16例化疗7d后的恶性血液病患者,以G CSF 15 0 μg 12h ,皮下注射,连用5d。应用流式细胞仪测定骨髓及外周血CD3 4+ 细胞和T细胞亚群。结果　①应用G CSF前,两者骨髓CD3 4+ 细胞有显著差异(P <0 0 1)。应用48h后,两者的骨髓CD3 4+ 细胞均较前有明显增加(P <0 0 1) ,两者之间仍存在明显差异(P <0 0 1)。两者的骨髓CD3 4+ 细胞高峰值出现在96h后,较应用前差异显著(P <0 0 1) ,两者之间无差异(P >0 0 5 )。②应用G CSF前,两者的外周血CD3 4+ 细胞无明显差异(P >0 0 5 )。应用72h后,两者外周血中CD3 4+ 细胞均较前有明显增加(P <0 0 1) ,两者之间无明显差异(P >0 0 5 )。96h后,两者的CD3 4+ 细胞达高峰,较应用前差异显著(P <0 0 1)。两者之间无明显差异(P >0 0 5 )。③应用G CSF对两者的T淋巴细胞亚群无明显影响。结论　应用G CSF动员外周血干细胞96h后,异体 自体造血干细胞移植供 受者的骨髓及外周血CD3 4+ 细胞均达到高峰,可适时采集外周血干细胞。Objective To explore the dynamic changes of CD34 + cells and T lymphocyte subsets and best time of harvesting peripheral blood stem cell when G-CSF was used for peripheral blood stem cell mobilization in donors and patients. Methods A total of 12 donors and 16 patients who received chemotherapy for 7 d were injected G-CSF of 300 μg/d to mobilize peripheral blood stem cells for 5 d and flow cytometry were used to detect the changes of CD34 + cells and T lymphocyte subsets everyday for 5 d. Results ① Before G-CSF treatment, there were obvious differences in bone marrow CD34 + cells between patients and donors (P<0.01). After 48-hour treatment, the amounts of CD34 + cells of both groups were obviously increased than before treatment (P<0.01). The highest amounts were reached after 96-hour treatment with significant difference between two groups (P<0.01). ② Before G-CSF treatment, there was no obvious difference between patients and donors in peripheral blood CD34 + cells. After 72-hour treatment, there was obvious increase in peripheral blood CD34 + cells in patients and donors (P<0.01), but there was no obvious difference between each other. After 96 h, the highest values were reached, which were higher than that before G-CSF treatment (P<0.01), but no difference between two groups. ③ There were no obvious changes in the donor's and patient's T lymphocyte subsets after using G-CSF for some days. Conclusion After mobilization for 96 h, the highest amounts of CD34 + cells in bone marrow or peripheral blood of patients and donors were reached and peripheral blood stem cells can be harvested.

关 键 词：重组人粒细胞集落刺激因子 骨髓 外周血 CD34^+细胞 T淋巴细胞亚群 

分 类 号：R331.14[医药卫生—人体生理学] R392.11[医药卫生—基础医学]