CPU-0213原料药的含量测定  

Studies on the Analytical Methods of CPU-0213

作　　者：王猛[1] 张钧寿[1] 张瑛[2] 

机构地区：[1]中国药科大学,南京210009 [2]东南大学,南京210096

出　　处：《江苏药学与临床研究》2005年第3期1-3,共3页Jiangsu Pharmacertical and Clinical Research

基　　金：国家自然科学基金资助项目(编号:30171078)

摘　　要：目的研究CPU0213含量测定的紫外分光光度法及反相高效液相色谱法。方法紫外分光光度法,检测波长258nm。高效液相色谱法,色谱柱汉邦ODS柱(4.6mm×150mm,5μm);流动相:甲醇醋酸醋酸钠缓冲液(pH3.6)(84∶16);检测波长258nm。结果紫外分光光度法,含量在2.52～20.16μg·ml-1浓度范围内,符合比尔定律。高效液相色谱法,在2.52～20.16μg·ml-1峰面积与浓度呈线性关系,r=0.9999。结论两种方法均能很好地进行CPU0213含量测定,测定结果基本一致。AIM To develop an method for the assay of new drug CPU-0213.METHODS HPLC and UV spectrophotometric method were used.The mobile phase consisted of methanol and acetate buffer solution (84∶16) for HPLC and detected at 258 nm both HPLC and UV methods.RESLUTS The calibration curve is linear in the range from 2.52 to 20.16 μg·ml -1 for the two methods.CONCLUSTION The results obtained by above two methods are in good agreement.

关 键 词：CPU-0213 含量测定 紫外分光光度法 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]