慢性乙型肝炎肝组织中星状细胞与肝纤维化关系的研究  

Relationship between fibrosis and hepatic stellate cell in liver tissues of patients with chronic hepatitis B

作　　者：陈超丽[1] 甄真[2] 冯翠玲[1] 刘晓平[1] 张新元[1] 何朝霞[1] 

机构地区：[1]石家庄市第五医院肝病临床中心科,石家庄050021 [2]河北医科大学第三医院感染科,石家庄050051

出　　处：《临床荟萃》2005年第12期661-663,共3页Clinical Focus

摘　　要：目的研究肝星状细胞(HSC)与肝纤维化和肝硬化的关系。方法检测60例慢性乙型肝炎患者肝功能及乙型肝炎e抗原(HBeAg),所有患者均进行了肝组织活检,其中S110例,S224例,S312例,S414例;采用免疫组织化学法,通过应用特殊的单克隆抗体(McAb)检测慢性乙型肝炎患者肝组织石蜡切片中α平滑肌肌动蛋白(αSMA)阳性细胞(即HSC)。结果苦味酸天狼猩红染色法胶原面积的百分比与肝纤维化分期有关(r=0.885,P<0.01);HSC与慢性乙型肝炎患者肝组织的纤维化分期呈正相关(r=0.761,P<0.01);HSC与慢性乙型肝炎患者肝组织中苦味酸天狼猩红染色法胶原面积的百分比正相关(r=0.854,P<0.01)。结论HSC与慢性乙型肝炎肝纤维化和肝硬化呈正相关。Objective To investigate the relationship between hepatic fibrosis and hepatic stellate cell(HSC). Methods To detect the contents of liver function and hepatitis B e antigen(HBeAg) in 60 cases of chronic hepatitis B(CHB), and to detect the hepatic biopsy in all cases. Ten cases were S 1, 24 cases were S 2, 12 cases were S 3, 14 cases were S 4. Alpha-smooth-muscle actin (alpha-SMA) positive cells (i.e.,HSC) were detected in paraffin-embeded liver tissue of biopsy patients with CHB by immunohistochemical method using specific monoclonal antibody(McAb). Results The percentage area of collagen by Sirius red stain was related to fibrosis staging(r=(0.885),P<(0.01)). There was positive correlation between HSC and fibrosis staging in liver tissues of patients with CHB (r=(0.761),P<(0.01)). There was positive correlation between alpha-SMA and collagen by Sirius red stain in liver tissues of patients with CHB(r=(0.854),P<(0.01)). Conclusion There was positive correlation between HSC and hepatic fibrosis,hepatic cirrhosis in the patients with CHB.

关 键 词：肝炎 乙型 慢性 肝硬化 星状细胞 细胞外基质 

分 类 号：R512.62[医药卫生—内科学]