反相高效液相色谱法测定复方薄荷脑滴鼻液中樟脑的含量  

Determination of Camphor in Compound Menthol Nose Drop by RP-HPLC

作　　者：田静 许亚玲 杨迪 

机构地区：[1]贵州省铜仁地区药品检验所,铜仁市554300

出　　处：《中国药房》2005年第12期944-945,共2页China Pharmacy

摘　　要：目的:建立以反相高效液相色谱法测定复方薄荷脑滴鼻液中樟脑含量的方法。方法:色谱柱为WatersNova-pakC18,流动相为三氯甲烷-甲醇-水(14∶52∶30),流速为1.0ml/min,检测波长为289nm,柱温为35℃。结果:樟脑检测浓度在0.8～5.6μg/ml范围内线性关系良好(r=0.9998),平均加样回收率为99.4%(RSD=0.7%)。结论:本方法专属性好、简便、快速、准确,可用于该制剂的质量控制。OBJECTIVE:To determine camphor in compound menthol nose drop by RP-HPLC.METHODS:Waters No-va-pak C 18 was taken as the chromatographic column,the mobile phase was composed of chloroform-methanol-water(14∶52∶30)with a flow speed at1.0ml/min and detection wavelength at289nm,the column temperature was35℃.RESULTS:The linear range for camphor was0.8～5.6μg/ml(r=0.9998)and the average recovery rate was99.4%(RSD=0.7%).CONCLUSION:The method is specific,convenient and accurate,which can be used for the quality control of the preparation.

关 键 词：反相高效液相色谱法 复方薄荷脑滴鼻液 樟脑 含量测定 

分 类 号：R927.2[医药卫生—药学]