2型糖尿病大鼠模型的制备及其验证  

Development and validation of the rat model with type 2 diabetes mellitus

作　　者：双卫兵[1] 王志慧 王东文[1] 吴博威[3] 

机构地区：[1]山西医科大学第一临床医学院泌尿外科,太原030001 [2]山西省大同市第三医院 [3]山西医科大学生理学教研室

出　　处：《山西医科大学学报》2005年第3期275-279,共5页Journal of Shanxi Medical University

基　　金：山西省自然科学基金资助项目(20021112);山西省归国留学人员基金资助项目(2002[16]39)

摘　　要：目的研制2型糖尿病大鼠模型。方法新生2d龄Wistar大鼠147只,随机分为造模组和对照组,造模组大鼠90mg/kg腹腔注射链脲佐菌素,断乳后再次25mg/kg追加一次,并应用高糖高脂膳食诱导建立2型糖尿病大鼠模型。结果从生化指标和形态学方面证实该方法可以成功制备2型糖尿病大鼠模型。结论该方法制备的2型糖尿病大鼠模型,造模时间短,成模率高,模型稳定。Objective To explore a method to develop the type 2 diabetes mellitus (T2DM) rat model. Methods Two-day-old Wistar rats (n=147) were divided into two groups: model and control groups. The modol group rats underwent intraperitoneal injection with 90 mg/kg streptozotocin (STZ). When they had ablacted they were injected with 25 mg/kg streptozotocin(STZ) again. Then these rats were fed the diets enriched with sucrose (20%) , lard(18%) and yolk powder(3%).Another group served as control group. Results Biochemical parameters and morphology proved that the methods could develop the T2DM rat model. Conclusion This method to develop T2DM rat model is high-efficient, stable and quick.

关 键 词：糖尿病 非胰岛素依赖型 疾病模型 动物 大鼠 Wistar 

分 类 号：R587.1[医药卫生—内分泌]