流式细胞仪分离精子法的研究进展  

Progress in Flow-Cytometrically Sorting the X- and Y-Chromosome Bearing Mammalian Sperm

作　　者：陆阳清[1] 张明[1] 卢克焕[1] 

机构地区：[1]广西大学动物繁殖研究所,南宁530005

出　　处：《生物技术通报》2005年第3期26-30,共5页Biotechnology Bulletin

基　　金："十五"国家重大科技专项(NO.2002BA518A01);国家自然科学基金(NO.30360073);国家科技攻关项目(NO.2002BA547C)资助

摘　　要：以X精子和Y精子的DNA含量差别为基础的流式细胞仪分离精子技术是有效的哺乳动物性别控制方法。目前,流式细胞仪分离精子的速度与上世纪90年代初期相比已提高了30多倍,以3000个/秒的常规速度分离X精子或Y精子的准确率可达到85%～90%。迄今,用分离精子人工授精已产下了数万头的动物后代,而分离人的精子也开始应用到了避免X染色体连锁遗传疾病的生殖医学工程中。Flow-cytometrically sorting the X-and Y-chromosome bearing sperm based on the measurement of sperm DNA is an effective way to preselect the sex of the mammalian offspring. The sorting rate today has been nearly thirty times of ten years ago. The accuracy of the sorted sperm is about 85% to 90% at a routine rate of 3000 live sperm for each sex. Thousands of mammalian offspring have now been produced via artificial insemination with sorted sperm, and sorted human sperm is also used in clinic to produce girls to avoid X-chromosome linked genetic diseases.

关 键 词：流式细胞仪 分离方法 精子 哺乳动物 性别控制 动物繁殖技术 

分 类 号：S814.3[农业科学—畜牧学]