人辅助生育技术培养环境的质量控制  

The quality control of culture condition in human assisted reproductive technology

作　　者：蔡霞[1] 程祖亨[1] 

机构地区：[1]新疆医科大学第一附属医院妇产科,乌鲁木齐830054

出　　处：《生殖医学杂志》2005年第3期159-162,共4页Journal of Reproductive Medicine

摘　　要：人类辅助生殖技术已成为当今治疗不育症的主要手段,为取得令人满意的种植率与临床妊娠率,体外受精(IVF)实验室的培养环境便成为生殖助孕工作者关注的重点。本文介绍几种常用于IVF实验室的生物测试质量控制方法。In vitro fertilization and embryo transfer procedures have been widely accepted throughout the world and are a useful technique for treating infertility. The major concern of an IVF laboratory is the high pregnancy rate which can only be achieved by producing high quality embryos. Many components used for IVF can impair the growth of human embryos. It is not surprising that even low levels of toxicants present during embryo culture will diminish the viability of the embryos. The quality control methods are used to detect toxins in related items that might directly or indirectly contact embryos. The optimal culture of human embryos in an IVF program demands the selection of non-embryo-toxic equipment and reagents. The toxicity of different reagents and materials used in human IVF program has been evaluated by using a range of bioassays that include the mouse embryo assay (MEA), the hamster sperm motility assay (HSMA), human sperm survival assay (HSSA), the amniotic fluid cell (AFC) and the hybridoma cell culture assay (HCCA). There are advantages and disadvantages in those mentioned methods. So, we can choose different method in different situation to test culture medium or components. This review introduced several bioassay methods to detect toxicity in IVF laboratory.

关 键 词：辅助生殖技术 培养环境 质量控制 生物测试 

分 类 号：R715.9[医药卫生—妇产科学]