电离辐射对松果腺细胞功能的影响  

Effect of ionizing irradiation on function of pineal gland cells

作　　者：王枫[1] 艾毅钦[2] 张铭[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,长春130021 [2]昆明医学院第一附属医院放疗科

出　　处：《中华放射医学与防护杂志》2005年第3期240-242,共3页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的研究电离辐射对松果腺细胞凋亡、cAMP和褪黑素(MLT)含量的变化规律及特点。方法采用流式细胞术检测松果腺细胞凋亡。采用放免分析法检测松果腺细胞中cAMP含量。采用高效液相色谱分析法检测松果腺细胞中MLT含量。结果小鼠受0.5～6GyX射线全身照射12h,随剂量增加松果腺细胞凋亡百分率增加;照射后24h,随剂量增加松果腺细胞中cAMP含量降低。小鼠受1～6Gy照射后12h,随剂量增加松果腺细胞合成和分泌MLT功能减弱。结论高剂量电离辐射对松果腺细胞功能有抑制作用。Objective To research the effects of ionizing radiation on apoptosis and pattern of changes in cAMP and melatonin (MLT) contents of pinealocytes. Methods The percentage of apoptosis of pinealocytes in mice was determined with flow cytometry. The contents of cAMP and MLT in pinealocytes were measured with radioimmunoassay and high preformance liquid chromatography, respectively. Results The percentage of apoptotic pinealocyte was observed in mice 12 hours after irradiation. The results showed that the percentage of cell apoptosis increased gradually after irradiation with 0.5-6.0 Gy X-rays. The content of cAMP in mouse pinealocytes reduced gradully after irradiation with 1.0-6.0 Gy, and was significantly lower after irradiation with 4.0-6.0 Gy than that in the control group (P<0.01). The content of MLT secreted by mouse pinealocytes decreased gradually 12 hours after irradiation with 1.0-6.0 Gy X-rays. Conclusion High dose ionizing radiation has inhibitory effect on the function of pinealocytes.;

关 键 词：电离辐射 细胞功能 高效液相色谱分析法 褪黑素(MLT) CAMP含量 松果腺细胞 流式细胞术检测 X射线全身照射 细胞凋亡 放免分析法 变化规律 细胞合成 抑制作用 百分率 24h 高剂量 小鼠 

分 类 号：R818[医药卫生—放射医学]