甲型副伤寒沙门菌株的脉冲电场凝胶电泳分型研究  

Study on the molecular typing of salmonella paratyphi A strains with pulsed field gel electrophresis

作　　者：陈忠妙[1] 徐景野[2] 郑官增[1] 江夏明[3] 金春光[2] 王琳娜[3] 何宏明[4] 刘令初 裘丹红[1] 郑伟[5] 王满琴[5] 何浙生[5] 

机构地区：[1]台州市疾病预防控制中心,318000 [2]宁波市疾病预防控制中心,315000 [3]温岭市疾病预防控制中心,317500 [4]玉环县疾病预防控制中心,317600 [5]浙江省医学科学院,310013

出　　处：《国外医学（流行病学．传染病学分册）》2005年第3期140-142,共3页Epidemiology Lemology Foreign Medical Sciences

摘　　要：目的探讨台州地区甲型副伤寒沙门菌株的流行病学特征及分型情况。方法用脉冲电场凝胶电泳(PFGE)方法进行分型。结果根据细菌染色体DNA的XbaⅠ酶切图谱,可将台州地区患者中分离的65株甲型副伤寒沙门菌分成9个PFGE型别(X1～X9)。来自温岭菌株有6个PFGE型,三门菌株有4个PFGE型,玉环菌株仅有2个PFGE型,其中X2为优势菌型。结论分型研究表明,甲型副伤寒沙门菌核型上虽在台州地区存在着县区性差异,但均以X2为主要流行菌型,从而提示了同一个克隆群内的菌株广泛传播可能是造成台州地区甲型副伤寒流行的主要原因。Objective To type and group the Salmonella paratyphi A strains isolated recent years in Taizhou to understand the epidemiological features caused by the pathogen. Methods Pulsed field gel electrophoresis of large restriction fragments of bacterial chromosomal DNA was used. Results The 65 isolates of Salmonella paratyphi A collected from the patients in recent years in Taizhou could be divided into 9 PFGE types(X1～X9) based on the size and number of restriction fragments and patterns,that were digested by XbaⅠ.Strains isolated from Wenling had 6 PFGE types.Strains isolated from Sanmen had 4 PFGE types,whereas strains isolated from Yuhuan showed only two types X2 and X8,of which the predominant type was X2. Conclusions Data indicated that although the distribution of PFGE types also varied in different counties in Taizhou,but X2 type as a main epidemic strain were detected in all counties.It was suggested that the widely spread of the same clone of strains might be the major cause in the prevalence of Salmonella paratyphi A in Taizhou.

关 键 词：脉冲电场凝胶电泳 分型研究 沙门菌株 甲型副伤寒沙门菌 台州地区 流行病学特征 染色体DNA 酶切图谱 XbaⅠ PFGE 流行菌型 E型 性差异 

分 类 号：R181.3[医药卫生—流行病学]