人巨细胞病毒对腮腺导管上皮细胞表型的影响及其机制  

Effects of HCMV on phenotypes of parotid duct epithelial cells and its mechanisms

作　　者：杨国嵘[1] 黄高昇[2] 白海[1] 王恩华[3] 宋继谒[3] 郭英[2] 王娟红[2] 梁蓉[2] 王哲[2] 

机构地区：[1]兰州军区兰州总医院全军血液病中心,730050 [2]第四军医大学病理教研室 [3]中国医科大学病理教研室

出　　处：《中华实验和临床病毒学杂志》2005年第2期132-134,i002,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助 (3 0 2 70 5 77)

摘　　要：目的　研究人巨细胞病毒(HCMV)感染对腮腺导管上皮细胞表型所产生的影响及其可能的机制。方法　用免疫组织化学方法研究腮腺巨细胞包涵体病石蜡包埋组织中巨细胞病毒立即早期抗原、广谱角蛋白、组织蛋白酶D等的表达。结果　腮腺导管上皮细胞感染HCMV后,作为上皮性标志物的角蛋白表达转为阴性,而组织蛋白酶D的表达转为强阳性。结论　HCMV感染后腮腺导管上皮细胞角蛋白丢失,其机制可能与组织蛋白酶D的表达增强有关。Objective To investigate the effects of HCMV infection on phenotypes of parotid duct epithelial cells and relative mechanisms. Methods The expressions of immediate early antigen of HCMV, pan cytokeratin and cathepsin D etc. were detected by immunohistochemical staining in tissues of parotid cytomegalic inclusion disease. Results Cytokeratin which acts as an epithelial marker became negative while staining of Cathepsin D was intensified in parotid duct epithelial cells after infected by HCMV. Conclusion It demonstrated that cytokeratin was lost through over-expression of Cathepsin D in parotid duct epithelial cells infected by HCMV.

关 键 词：人巨细胞病毒 腮腺导管 细胞表型 组织蛋白酶D 免疫组织化学方法 巨细胞包涵体病 石蜡包埋组织 感染HCMV 上皮性标志物 上皮细胞 早期抗原 蛋白表达 蛋白丢失 表达增强 角蛋白 强阳性 感染后 

分 类 号：R512.99[医药卫生—内科学]