HIV/AIDS患者肝组织HIV-1 p24抗原的免疫组化检测  

Immunohistochemical evidence for HIV-1 infection in the liver of HIV-infected patients

作　　者：姜天俊[1] 赵敏[1] 赵景民[2] 周光德[2] 潘登[2] 王健[3] 张云辉[1] 周志平[1] 

机构地区：[1]解放军第三○二医院感染一科,北京100039 [2]解放军第三○二医院病理科,北京100039 [3]中国中医研究院基础所艾滋病研究室

出　　处：《中华实验和临床病毒学杂志》2005年第2期152-154,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　检测HIV抗原标志物在肝组织的表达情况,探讨HIV AIDS患者肝损伤发生的可能机制。方法　采用免疫组织化学方法对14例HIV AIDS患者的肝活检组织切片进行HIV 1p2 4抗原检测,比较不同病变肝组织中HIV 1p2 4抗原的表达数量。结果　在14例肝组织切片的Kupffer细胞、内皮细胞、肝细胞均发现HIV 1p2 4抗原表达;阳性肝细胞计数分析显示,表达数量随病变严重程度而增加(P <0 0 5 )。结论　HIV能够在HIV AIDS患者的肝脏内表达,并且可能参与肝细胞的凋亡。Objective To detect p24 antigen of human immunodeficiency virus (HIV)-1 in the liver biopsy specimens of patients with HIV infection. Methods Liver biopsy samples from 14 patients with HIV/AIDS (11 man, 3 women; age range 27-52 years; infection time range 8-13 years) were examined by immunohistochemistry prospectively. Results Intracellular expression of HIV-1 p24 antigen was detected in Kupffer cells, endothelial cells and hepatocytes. There were more HIV-positive liver cells in the patients with severer liver damage than those with milder liver damage (t=2.5189, P=0.0270). Conclusion These findings indicate that HIV-1 could replicate in the liver of HIV-infected patients and might be related to the liver cells apoptosis.

关 键 词：HIV/AIDS患者 P24抗原 免疫组化检测 KUPFFER细胞 HIV-1p24 免疫组织化学方法 活检组织切片 病变严重程度 抗原标志物 肝组织切片 表达情况 可能机制 抗原检测 内皮细胞 抗原表达 细胞计数 肝细胞 肝损伤 肝脏内 

分 类 号：R512.91[医药卫生—内科学] R373.9[医药卫生—临床医学]