丙型肝炎病毒体外感染Hep G_2细胞的研究  

In vitro study on Hep G_2 cell infected by hepatitis C virus

作　　者：陈瑞烈[1] 贺永文[2] 高勇[2] 李淑莉[2] 杨小铭[2] 罗端德[2] 

机构地区：[1]广东省汕头市第二人民医院感染科,515011 [2]华中科技大学同济医学院附属协和医院传染科

出　　处：《中华实验和临床病毒学杂志》2005年第2期156-158,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　建立接近体内自然感染状态并能长期复制的丙型肝炎病毒(HCV)感染细胞模型。方法　用HCVRNA阳性血清感染人肝癌细胞株HepG2 ,培养6 0d ,用套式逆转录聚合酶链反应(nestedRT PCR)检测培养细胞及上清中正、负链HCVRNA。结果　HepG2 在感染后第2～30天,细胞中可以间断检出HCV正链RNA ;负链RNA在感染后第3～30天可以间断检出,检出率与正链RNA接近。在第31～6 0天仍可间断检出HCVRNA正、负链,但检出率逐渐降低。HepG2 培养上清中HCV正链RNA感染后也呈间断阳性,检出率与细胞内正链RNA基本一致。培养上清中均未检出HCV负链RNA。结论　HepG2 细胞对HCV易感,并能支持HCV长期复制,HepG2 细胞是较为理想的HCV体外感染细胞模型。Objective To establish hepatitis C virus (HCV) infected cell model which is similar to the infection in vivo and can support HCV to replicate for a long time. Methods After infected with HCV-positive serum, Hep G 2 cells were cultured for 60 days. Nested RT-PCR was used to detect plus and minus HCV RNA in cultured cells and supernatants. Results Plus HCV RNA was detected intermittently in Hep G 2 cells during 2-30 days, minus HCV RNA was detected during 3-30 days after infection, the detection rate was similar to plus HCV RNA. Plus and minus HCV RNA can be still intermittently detected during 31-60 days after infection. However, the detection rate gradually declined. Plus HCV RNA was also found intermittently positive in the supernatant, and the detection rate was consistent to that in cells. Minus HCV RNA was not detected in the supernatant.Conclusion Hep G 2 cells were susceptible to HCV, and could support HCV to replicate for a relatirely long time. Hep G 2 is an ideal HCV infection cell model.

关 键 词：G2细胞 体外感染 HEP 丙型肝炎病毒(HCV) 人肝癌细胞株 聚合酶链反应 RNA 细胞模型 培养上清 感染后 检出率 感染状态 血清感染 培养细胞 负链 正链 逆转录 细胞内 复制 阳性 

分 类 号：R373[医药卫生—病原生物学]