酚氧化酶在家蚕血淋巴中的活力分布及影响因素的研究  

The research on the activity of phenoloxidase in the haemolymph of Bombyx mori and its in fluence factors

作　　者：陈文柱[1] 李兵[1] 贡成良[1] 沈卫德[1] 

机构地区：[1]苏州大学蚕桑研究所苏州大学生命科学学院,苏州215006

出　　处：《江苏蚕业》2005年第2期12-15,共4页Jiangsu Sericul Ture

基　　金：江苏省高校自然科学研究计划项目资助(No.01KJB180002)国家自然基金项目资助(No.30471309)

摘　　要：昆虫血淋巴黑化的形成由激活酚氧化酶原的级联系统所引发,酚氧化酶在昆虫体液免疫中起着非常重要的作用。本试验以家蚕3龄末期开始每天抽取血淋巴制成粗酶液,以L-DOPA为底物,测定各龄期活力分布规律,并且通过改变酚氧化酶与底物反应的条件,发现温度与PH值对酚氧化酶添力具有很大的影响。The melanism of blood lymph of insects is casused by the cascade system which activates prophenoloxidase. Phenoloxidase palys very important roles in the body fluid immune system of insects. In this experiment, blood lymph of third instar larvae of Bombyx mori was extracted to make into crude enzyme fluid every day from the telophase and L-DOPA taken as a substrate to measure the activity distribution law at every instar of Bombyx mori. After changing the conditions of the reaction between phenoloxidase and the substrate , it was found that temperature and PH had great effects on the activity of phenoloxidase.

关 键 词：酚氧化酶 家蚕 血淋巴 活力分布 体液免疫 温度 PH值 

分 类 号：S881.24[农业科学—特种经济动物饲养]