全反式视黄酸在骨髓间充质干细胞向神经元分化中的作用研究  

THE EFFECT OF ALL-TRANS RETINOIC ACID ON THE DIFFERENTIATION OF MARROW STROMAL STEM CELLS INTO NEURONS

作　　者：卓本慧[1] 李廷玉[1] 江和碧[1] 瞿平[1] 刘洋[1] 

机构地区：[1]重庆医科大学附属儿童医院营养研究中心,重庆400014

出　　处：《营养学报》2005年第3期189-192,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30070382)

摘　　要：目的:探讨全反式视黄酸(ATRA)在骨髓间充质干细胞(MSCs)向神经元分化中的作用及机制。方法:大鼠MSCs在体外培养5～7代后,用0.5μmol/L的ATRA预诱导24h,再换用改良的神经细胞培养基(MNM)作用18h;对照组不用ATRA预诱导,直接用MNM培养。免疫组化检测不同组别巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、神经元特异性核心抗原(NeuN)和微管相关蛋白-2(MAP-2)的表达情况;ATRA作用前后检测细胞增殖周期和视黄酸受体β(RARβ)的变化。结果:1.ATRA预诱导组巢蛋白、NSE、NeuN和MAP-2的阳性比例明显高于对照组。2.ATRA作用前后,MSCs细胞增殖周期无明显改变。3.MSCs不表达RARβ,用ATRA预诱导细胞后,RARβ表达增加,阳性比例为(20.3±4.2)%。结论:ATRA可能通过RARβ介导靶基因的转录,促进MSCs向神经元分化。Objective:To investigate the effect of all-trans retinoic acid (ATRA) on the differentiation of marrow stromal stem cells(MSCs)into neurons. Methods: Rat MSCs were expanded as undifferentiated cells in culture for 5-7 passages and pretreated with ATRA for 24 h, then induced by modified neuronal medium (MNM) for 18 h. The control cells were directly cultured in MNM without ATRA pretreatment. Immunocytochemistry was used to detect the expression of nestin, neuron-specific enolase (NSE), neuron-specific nuclear protein (NeuN) and microtuble-associated protein (MAP-2) in the experimental groups and the control cells. The change of cell proliferative cycles and the expression of retinoic acid receptor beta (RARβ) were detected before and after ATRA treatment. Results: 1. The expression of nestin, NSE, NeuN and MAP-2 was much higher than that of the control group. 2. No changes were found in the cell proliferative cycles before and after ATRA treatment. 3. No expression of RARβ was found in MSCs; but positive expression of RARβ was detected afer 24 h of ATRA treatment and it was (20.3±4.2)%. Conclusion: ATRA can activate gene transcription via nuclear receptor RARβ and promote MSCs transdifferentiation into neurons.

关 键 词：视黄酸 骨髓间充质干细胞 神经元 

分 类 号：Q254[生物学—细胞生物学] R977.21[医药卫生—药品]