胡萝卜与川西獐牙菜不对称体细胞杂种的核/质基因组特征  

The Characteristics of Nuclear/Cytoplasmic Genomes of Asymmetric Somatic Hybrid of Daucus carota var. sativa and Swertia mussotii

作　　者：李子东[1] 邵菲[2] 蔡云飞[1] 向凤宁[1] 夏光敏[1] 

机构地区：[1]山东大学生命科学学院,济南250100 [2]中国农业大学农业生物技术国家重点实验室,北京100094

出　　处：《植物生理与分子生物学学报》2005年第3期254-260,共7页Journal Of Plant Physiology and Molecular Biology

基　　金：山东省自然科学基金重点项目(No.Z2001D01);国家自然科学基金项目(No.30470154)资助。~~

摘　　要：胡萝卜(Daucuscarotavar.sativa)原生质体与经260μW/cm2紫外线照射的川西獐牙菜(Swertiamussotii)原生质体用PEG法诱导融合。对融合再生的克隆的5SrDNA间隔序列和RAPD分析结果得知,各再生克隆均存在双亲的核DNA及重组DNA。杂种的核基因组组成以胡萝卜(受体)为主,供体川西獐牙菜DNA谱带较少;UV照射剂量对体细胞杂种核基因组组成没有明显的影响。进一步对再生克隆叶绿体DNA的SSR分析表明,杂种细胞中双亲叶绿体基因组随机分离并发生重组。Protoplasts of Swertia mussotii irradi-ated by ultra-violet light (UV) 260 μW/cm2 werefused with protoplasts of Daucus carota var. sa-tiva using the PEG method. Analysis of 5S rDNAspacer sequences (Fig.1) and random amplifiedpolymorphic DNA (RAPD) (Table 2, Fig.2)showed the presence and combination of nuclearDNA from both parents. All hybrids mianly con-tained genomes of D. carota (receptor) RAPDbands, and a few donor RAPD bands of S. mussotii(Tables 2, 3; Fig.2). There is no obvious relation-ship between the nuclear DNA composition was notaffected by UV-dosage (Table 4). SSR analysis of chloroplast DNA of the hybrid clones further re-vealed that cpDNAs of the parents were integratedand recombined in the hybrids randomly (Fig.3).

关 键 词：胡萝卜 川西獐牙菜 紫外线处理 不对称体 细胞杂交 核/质基因组 

分 类 号：S334[农业科学—作物遗传育种]