Triton X-100对70℃处理后光系统I颗粒耗氧速率的影响  

Effects of Triton X-100 on the Oxygen Uptake Rate of Photosystem I Particles Treated at 70℃

作　　者：陈伟[1] 阳振乐[1] 李良璧[1] 匡廷云[1] 

机构地区：[1]中国科学院植物研究所光合作用与环境分子生理学重点实验室

出　　处：《植物生理与分子生物学学报》2005年第3期298-304,共7页Journal Of Plant Physiology and Molecular Biology

基　　金：中国科学院创新工程方向性项目(No.KSCXZ-SW-31260);国家自然科学基金项目(No.39890390)资助。~~

摘　　要：比较了70℃10min处理前后和加与不加TritonX-100时光系统I颗粒的耗氧速率、荧光光谱和吸收光谱等。70℃处理后光系统I颗粒的耗氧速率明显降低,TritonX-100可以恢复其耗氧速率。在TritonX-100存在时,光系统I颗粒耗氧速率的急剧上升是光系统I核心复合物和捕光色素蛋白复合体I分离后产生的单线态氧引起的。The characteristics including oxygenuptake rates, fluorescence spectra and absorp-tion spectra of photosystem I particles with orwithout Triton-X 100 treatment before or afterthe incubation at 70℃ for 10 min were compared.The oxygen uptake rates of photosystem I par-ticles decreased after being incubated at 70℃for 10 min, which could be recovered by the ad-dition of Triton-X 100 (Table 1). Singlet oxygenwas formed when the light-harvesting complexI was separated from the core complex of photo-system I, which resulted in high oxygen uptakerate (Table 2). There was much difference in thefluorescence spectra of photosystem I particles between photosystem I particles treated withTriton-X 100 after the incubation at 70℃ for 10min or not, which implies the ability of Triton-X100 to promote the recovery of photosystem Iparticles after the incubation at 70℃ for 10 min(Fig.2 and Table 2).

关 键 词：光系统Ⅰ颗粒 耗氧速率 TRITONX-100 高温处理 单线态氧 光合作用 

分 类 号：Q945.1[生物学—植物学]