微量稀释法检测氟康唑和伊曲康唑对白念珠菌的敏感性  

Observing the Susceptibility of Candida Albicans Challenge with Fluconazole and Itraconazole by M27-A Microdilution Method

作　　者：贺志彬[1] 廖万清[2] 徐红[2] 温海[2] 

机构地区：[1]中国人民解放军第187医院皮肤科,海口571159 [2]上海长征医院皮肤科,上海200003

出　　处：《中国中西医结合皮肤性病学杂志》2005年第2期71-73,共3页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的了解氟康唑和伊曲康唑对白念珠菌敏感性。方法采用NCCLS公布的M27-A方案微量稀释法,对19株白念珠菌临床分离株和3株标准株进行氟康唑和伊曲康唑药物的敏感性观测。结果19株临床分离株发现氟康唑耐药株7株,敏感株8株,剂量依赖的敏感菌株4株;伊曲康唑耐药株9株,敏感株8株,余2株为剂量依赖的敏感菌株;3株标准株结果符合。结论M27-A方案微量稀释法适合于临床检测白念珠菌的药物敏感性,氟康唑和伊曲康唑在白念珠菌中存在较高的耐药率和交叉耐药现象。Objective To investigate the susceptibility of Candida albicans cultured with Fluconazole and Itraconazole. Methods M27-A microdilution method based on NCCLS was used to determined the susceptibility of 19 Candida albicans isolates and 4 ATCC cultured with Fluconazole and Itraconazole. Results 7 Fluconazole-resistance isolates, 8 susceptible isolates, 4 susceptible dose dependent(SDD) isolates, 9 Itraconazole-resistance isolates, 8 susceptible isolates, and 2 SDD isolates were detected from 19 clinical Candida albicans isolates. Conclusion It suggested that M27-A method based on NCCLS is suitable to examin the susceptibility of Candida albicans, and the obvious resistance to Fluconazole and Itraconazole of Candida albican and cross-resistance between Fluconazole and Itraconazole were found in this study.

关 键 词：白念珠菌 氟康唑 伊曲康唑 药物敏感性 

分 类 号：R519.3[医药卫生—内科学]