根癌农杆菌转化谷子细胞早期的细胞生物学研究  

Cell Biological Study on the Early Process of Transformation of Millet(S. italica)Cells With Agrobacterium tumefaciens

作　　者：余迪求[1] 许耀[1] 李宝健[1] 

机构地区：[1]中山大学生物工程研究中心

出　　处：《中山大学学报（自然科学版）》1994年第2期10-17,共8页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家高技术发展计划(863)资助项目

摘　　要：本文利用光镜、电镜和电镜放射自显影技术观察了有效高活性根癌农杆菌转化谷子培养细胞早期的细胞生物学过程。经复合人工信号物质和天然复合敏感抽提物处理的农杆菌可向不敏感的谷子细胞表面移动、聚集并附着，在靠近谷子细胞一极，菌体形成颗粒状内含物，并表现为多型态，在￣３Ｈ—ＴｄＲ放射性标记的高活性农杆菌与谷子细胞的共培养过程中，可依次在谷子细胞的细胞质周缘、核内及核仁区域观察到Ｔ－ＤＮＡ银颗粒的分布。By light and electron microscopic as well as EM autoradiography technique,we observed systematically the early cell biological process of the transformation of mil-let(S,italica)cells with high-effective Agrobacterium tumefaciens. The Agrobacteriapretreated with complex synthetic signal material(8SSM)and natural sensitive extrac-tions(NSE)could move toward,gather and attach to non-sensitive millet cell sur-faces.The electron microscopic observation demonstrated the bacteria became pleomor-phic and formed granular inclusions that migrated to the polar position which nearedplant cells,During co-cultivation between the high-effective bacteria cells labled by￣3H-TdR and millet cells,we observed that T-DNA silver grains exist successively inthe cytoplasm,nuclei and nucleoli regions.

关 键 词：根癌农杆菌 谷子 细胞生物学 

分 类 号：Q949.714.2[生物学—植物学]