配位色谱法分离纯化白果内酯  被引量:2

Preparative Separation of Bilobalide by Coordination Chromatography

在线阅读下载全文

作  者:潘见[1] 徐涛[1] 

机构地区:[1]合肥工业大学农产品生物化工教育部重点实验室,合肥230069

出  处:《药物分析杂志》2005年第6期614-617,共4页Chinese Journal of Pharmaceutical Analysis

基  金:国家自然科学基金重点项目(20136020)

摘  要:目的:建立白果内酯的配位色谱分离纯化方法。方法:10g 白果内酯初提物经柱前与0.01mol·L^(-1)配位剂-硼砂反应,0.1 mol·L^(-1)盐酸调 pH 到5.0左右,常压硅胶柱层析,石油醚-乙酸乙酯混合溶剂梯度洗脱。结果:Na_2B_4O_7配位色谱法所得白果内酯的纯度为82.1%和76.9%;回收率为75.5%和72.1%。较普通色谱法纯度提高7.4%-12.6%,回收率提高11.9%-15.3%。结论:本方法操作简便,产品纯度和得率较高,可用于白果内酯的分离纯化。Objective:To develop a method for the isolation and purification of bilobalide according to the principle of coordination chromatography.Method:The bilobalide Extract(10g)was treated with 0.01 mol·L^(-1) sodium bo- rate.The mixture was adjusted to pH 5.0 by 0.1 mol·L^(-1) hydrochloric acid.After absorption of the bilobalide Extract onto silica gel,elution was carried out with a gradient elution of the ratio of ethyl acetate to petroleum ether. Results:The purities of bilobalide separated by borate coordination chromatography were 82.1% and 76.9%,which were 12.6% and 7.4% higher than the blank column chromatography,recoveries were 75.5% and 72.1%,which were 15.3% and 11.9% higher than the blank column chromatography.Conclusion:The developed method is sim- ple,reproducible and easy to operate.

关 键 词:白果内酯 色谱法 配位 分离纯化方法 硅胶柱层析 梯度洗脱 混合溶剂 乙酸乙酯 产品纯度 回收率 石油醚 

分 类 号:R284.1[医药卫生—中药学] R735.704[医药卫生—中医学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象