高效液相色谱法测定复方吲哚美辛栓中硫酸沙丁胺醇的含量  

HPLC Determination of Salbutamol Suffate in its Compound Suppositories

作　　者：林冬青[1] 佟艳华[1] 车宝泉[1] 于美仙[1] 

机构地区：[1]北京市药品检验所,100035

出　　处：《药物分析杂志》2005年第6期709-710,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立复方吲哚美辛栓中硫酸沙丁胺醇含量测定的反相高效液相色谱法。方法:采用十八烷基键合硅胶色谱柱(250mm×4.6mm,5μm),以乙腈-0.25%庚烷磺酸钠溶液(2∶8),用磷酸调节 pH 值为3.2为流动相;检测波长220nm。结果:线性范围为2.5-126μg·ml^(-1),r=0.99999;平均回收率为100.9%,RSD 为0.99%。结论:本方法灵敏度高,操作简便、可靠。Objective:to set up a HPLC method for the determination of Salbutamol Sulfate in Indometacin Patches。Methods:Dia- monsil C_(18) column(250 mm×4.6mm,5um)was used the stationary phase,acetonitrile-0.25% sodium heptanesulfonate(2:8)and was used the mobile phase。The detection wavelength was 220 nm。Results:The method was proved to the linear in the range of 2.5 μg- 126 μg·ml^(-1),the regression coefficient is 0.9999。The Recovery of method was 100.9%,RSD0.99%(n=9)。Conclusion:The method is high sensitivity、simple、reliable

关 键 词：复方吲哚美辛栓 硫酸沙丁胺醇 反相高效液相色谱法 平均回收率 含量测定 检测波长 线性范围 色谱柱 磺酸钠 流动相 pH值 RSD 灵敏度 

分 类 号：R169.42[医药卫生—公共卫生与预防医学] R974.3