RP-HPLC法同时测定颠茄磺苄啶片中SMZ和TMP的含量  

RP-HPLC Determination of Belladonna Sulfamethoxazole and Trimerhoprim tablets

作　　者：丁建[1] 朱斌清 王玲娜[3] 

机构地区：[1]江苏省常州药品检验所,常州213003 [2]常州康普药业有限公司,常州213172 [3]苏州大学,苏州215001

出　　处：《药物分析杂志》2005年第6期715-716,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:测定颠茄磺苄啶片剂中2种组分的含量。方法:采用 Zorbax SB-C_(18)色谱柱(150mm×4.6mm,5μm),以 pH 为2.5的0.025moL·L^(-1)磷酸盐缓冲液-乙腈(75∶25)为流动相,流速为1.0mL·min^(-1),检测波长为230nm,进样量20μL,用外标法测定。结果:SMZ 在16～160μg·mL^(-1)范围内线性关系良好(r=0.9999),平均回收率为99.75%,RSD 为0.35%,TMP 在3.2～32μg·mL^(-1)范围内线性关系良好(r=0.9999),平均回收率为100.7%,RSD 为0.51%。结论:本方法简便、可靠,特别是比原方法更准确地测定颠茄磺苄啶片中 TMP 的含量。Objective:To determine Belladonna Sulfamethoxazole and Trimerhoprim tabalets by RP-HPLC. Methods:A Zorbax SB-C_(18)column was used.The mobile phase was phosphoric buffer(pH2.5,0.025 moL·L^(-1)) -acetonitrile(75:25)and the flow rate was 1.0 mL·min^(-1).The UV detection wavelength was 230nm.Results: The SMZ curve was linear in the range from 16μg·mL^(-1) to 160μg·mL^(-1)(r=0.9999)and the average recovey was 99.75%(RSD=0.35%),The TMP curve was linear in the range from 3.2 μg·mL^(-1)to 32 μg·mL^(-1)(r= 0.9999)and the average recovey was 100.7%(RSD=0.51%).Conclusion:The method is simple and reliable, specially,the method was more accurate for the determination of TMP in Belladonna Sulfamethoxazole and Trimer- hoprim tablets.

关 键 词：颠茄磺苄啶片 TMP SMZ HPLC法 含量 同时测定 平均回收率 RP C18色谱柱 磷酸盐缓冲液 线性关系 检测波长 RSD 流动相 外标法 

分 类 号：R927.3[医药卫生—药学] R978.2