CHOD-PAD法测定蛋黄卵磷脂中胆固醇的含量  

CHOD-PAD Determination of Cholesterol in Lecithin from Egg Yolk

作　　者：陈晓楠[1] 黄明刚[1] 宋航[1] 付超[1] 孟衍建[2] 杨丹[2] 

机构地区：[1]四川大学制药与生物工程系,成都610065 [2]四川生殖卫生学院附属医院,成都610041

出　　处：《药物分析杂志》2005年第6期729-730,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:测量精制蛋黄卵磷脂中胆固醇的含量。方法:采用 CHOD-PAD 法。结果:胆固醇浓度在0.431-2.585mmol·L^(-1),r=0.9991,重复性实验 RSD 为2.6%,精密度实验 RSD 为0.42%,回收率为97.3%。检测限低于0.431mmol·L^(-1),可满足卵磷脂中胆固醇的检测需要。结论:方法操作简便,精密度好,结果准确可靠。Objective:To determine the cholesterol amount in lecithin from egg yolk.Method:Method of CHOD- PAD.Results:The standard curve is linear in the range of 0.431-2.585 mmol·L^(-1)and the correlation coefficient is 0.9991.The RSDs for the repetitionand for the precisionis 2.6% and 0.42% respectively.The recovery is 97.3%.The lowest concentration to be measurable is lower than 0.431 mmol·L^(-1).It is suitable for determination of cholesterolin lecithin.Conclusion:The proposed method is easy to use with highly accuracy and reliability.

关 键 词：蛋黄卵磷脂 PAD 含量 测定 胆固醇浓度 精密度实验 RSD 重复性 回收率 检测限 

分 类 号：R971.1[医药卫生—药品] R197.39[医药卫生—药学]