采用纤维连接蛋白结合法分离人外周血单核细胞  

USE OF FIBRONECTN BINDING METHOD FOR ISOLATINGHUMAN PERIPHERAL BLOOD MONDCYTES

作　　者：侯凡凡[1,2] 叶任高[1,2] 

机构地区：[1]中山医科大学肾脏研究所 [2]现在第一军医大学南方医院肾病实验室

出　　处：《中山医科大学学报》1994年第4期308-310,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：国家教委博士点基金

摘　　要：利用人单核细胞表面有纤维连接蛋白受体的特点，我们建立了一种分离、纯化人外周血单核细胞的方法。该法制备的细胞悬液单核细胞占９７％以上，纯度明显优于Ｆｉｃｏｌｌ－Ｈｙｐａｑｕｅ密度梯度离心法。单核细胞获得率近５倍于传统粘附法，且细胞活性保持在９７％以上，是一种分离人外周血单核细胞的简单而有效的方法。simple and efficient method of monocyte separation was developed based on the fact that fibronectin receptors are present on the membrane of human monocytes. The percent of monocytes was beyond 97% in cells obtained by this method , it was significantly high than that obtqined by Ficoll-Hypaque centrifugation. The yield of monocytes was 5 times more than that with classical adherance method , and the cell viability was always excellent (> 97% ) . We concluded that this method is a simple and useful technique in separating human peripheral blood monocytes.

关 键 词：单核细胞 纤维连接蛋白 分离技术 

分 类 号：R446.8[医药卫生—诊断学]