胰岛素耐受的HepG2细胞模型建立  

Establishment of Insulin-resistant HepG2 Cell Model Induced by High Concentration of Insulin

作　　者：陈秋[1] 夏永鹏[1] 邱宗荫[1] 

机构地区：[1]重庆医科大学药学系,重庆400016

出　　处：《细胞生物学杂志》2005年第3期334-338,共5页Chinese Journal of Cell Biology

基　　金：重庆市自然科学基金资助项目(No.20038012)~~

摘　　要：采用高胰岛素体外诱导培养HepG2细胞,建立胰岛素耐受的细胞模型。将HepG2细胞置于5×10-7mol/L胰岛素培养液中24h,采用3H-D-葡萄糖掺入试验及残余125I-胰岛素结合率测定等方法观察高胰岛素对HepG2细胞葡萄糖摄取率及其胰岛素受体数目和功能的影响。高胰岛素诱导培养的HepG2细胞葡萄糖掺入率及残余125I-胰岛素结合率明显低于未用高胰岛素诱导的HepG2细胞(对照细胞)。将高胰岛素诱导培养的HepG2细胞置于不含胰岛素的培养液中60h,其细胞葡萄糖摄取率仍明显低于对照细胞。将HepG2细胞置于5×10-7mol/L胰岛素环境中24h,该细胞对胰岛素的生物学效应产生抵抗,其胰岛素抵抗状态可维持60h。To establish insulin resistant HepG2 cell model induced by high concentration of insulin in vitro culture. HepG2 cells were incubated with 5×10-7 mol/L insulin for 24 hours, washed with DMEM and finally with pre-cold PBS. The residual 125I-insulin binding and the insulin-stimulated 3H-D-glucose incorporation were determined. It was demonstrated that the residual 125I-insulin binding and incorporation rate of 3H-D-glucose in insulin-resistant HepG2 cells were decreased significantly than that in control cells. Insulin-resistant HepG2 cells were free from stimulation for 60 hours by insulin, but the incorporation rate of 3H-D-glucose were remaining lower than that in control cells. HepG2 cells exposed to 5×10-7 mol/L insulin for 24 hours were able to induce a state of insulin resistance and reproduce a insulin-resistant cell model. The state of insulin resistance could be maintained for 60 hours. The method is simple, practicable and reliable.

关 键 词：胰岛素 HEPG2细胞 细胞模型 胰岛素耐受 受体 

分 类 号：R965.1[医药卫生—药理学]