蝎毒多肽提取物的抗血管生成作用  

The Effect of Polypeptide Extract from Scopion Venom on Angiogenesis in Vitro

作　　者：张维东[1] 崔亚洲[2] 武利存[1] 贾青[1] 宋守芹[1] 王朝霞[1] 董强[1] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250062 [2]山东省医药生物技术研究中心,济南250062

出　　处：《细胞生物学杂志》2005年第3期343-346,共4页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.30371841);山东省自然科学基金(No.2003C02)资助项目~~

摘　　要：用不同浓度的东亚钳蝎素的多肽提取物PESV(4￣20μg/ml)作用于人脐静脉内皮细胞(HUVEC),观察HUVEC增殖活性和凋亡变化,增殖活性检测采用BrdU掺入的ELISA法,凋亡水平和凋亡相关基因Bcl-2和Bax表达的检测采用流式细胞术检测;用鸡胚尿囊膜(CAM)显示PESV对血管生成的抑制作用。结果显示,PESV抑制HUVEC的增殖,而对乳腺癌细胞MDA-MB-231的增殖无明显影响;PESV作用72h后,HUVEC凋亡相关基因Bcl-2表达降低,Bax表达增加,凋亡细胞比例增至10.5%,明显高于对照组;0.5mgPESV能明显抑制CAM新生血管的形成。因此,PESV具有良好的体外抗肿瘤血管生成活性,PESV作为一种肿瘤血管抑制剂的天然药物来源,其有效成分和药理作用有待进一步研究。The study was designed to evaluate the anti-angiogenic activity in vitro of PESV, a peptide extract from scorpion venom. Human umbilical vascular endothelial cell (HUVEC) was used to test the anti- angiogenic effect of PESV in vitro. HUVEC proliferation was detected by the BrdU cell incorporation ELISA. Apoptosis level and expression of Bcl-2 and Bax protein of HUVEC was determined by flow cytometry. Chicken embryo chorioallantoic membrane (CAM) assay was used to determine the effect of PESV on neovascularization. PESV exhibited potent anti-proliferative and apoptosis-induced activity against HUVEC in vitro. In addition, PESV demonstrated preferential inhibition of endothelial cells. PESV also demonstrated suppression of neovascularization in the CAM assay. Our data suggest that PESV is of potent anti-angiogenic activity in vivo. PESV could be regarded as a candidate for tumor angiogenesis inhibitor (TAI) and a potent chemotherapeutic agent of malignant tumors, and its component should be further analyzed.

关 键 词：蝎毒 多肽提取物 抗血管生成作用 人脐静脉内皮细胞 细胞增殖 细胞凋亡 肿瘤 

分 类 号：R96[医药卫生—药理学]