渗透胁迫下白花柽柳cDNA文库的构建及EST分析  

Construction and Analysis of Tamarix albiflonum M. T. L iu. cDNA Library under Osmosis Stress

作　　者：蔡勤安[1] 王玉成[2] 张道远[3] 董玉芝[1,4] 

机构地区：[1]新疆农业大学林学院 [2]东北林业大学 [3]中国科学院新疆生态与地理研究所 [4]东北林业大学,黑龙江哈尔滨150040

出　　处：《生物技术》2005年第3期21-25,共5页Biotechnology

基　　金：中国科学院新疆生态与地理研究所创新领域前沿课题(KZCX-XJ02-05)项目资助

摘　　要：目的:寻找渗透胁迫相关基因EST片断。方法:构建白花柽柳cDNA文库,对文库阳性克隆随机测序,并进行网上比对分析。结果:该cDNA文库克隆的重组率大于95%,插入片段主要集中在250～1000bp之间,并获得了如脯氨酸丰富蛋白基因,钙依赖蛋白激酶基因,丝氨酸苏氨酸激酶基因,过氧化氢酶基因,膜转运蛋白等基因。结论:渗透胁迫基因是多基因参与的系统调控过程,它们涉及了植物细胞壁合成、信号传递、基因调控、氧化物质清除、渗透调节物质的转运等方面。Objective:To find the EST of Osmosis stress-associated genes. Method:a cDNA library was constructed from leaves of Tamarix albiflonum M. T. L iu .treated with osmosis stress. The picked positive clones of the library was randomly sequenced and analysed through BLASTX.Results:The rate of recombination was above 95% and the size of most inserts were 250～1000bp in the library and some genes were identified such as proline rich protein, calcium dependent protein kinase, serine/threonine-protein kinase, Catalase, integral membrane transporter protein etc.Conclusion:Osmosis stress-associated genes were multiple-gene that participated in systematic regulation process. The function of these genes included cell wall synthesis,signal component,regulatory protein and antioxidant enzyme.

关 键 词：白花柽柳 渗透胁迫 CDNA文库 表达序列标签 

分 类 号：Q75[生物学—分子生物学] Q785