深圳献血员HBsAg阴性HBVDNA阳性的追踪分析  

作　　者：王飞[1] 尚桂芳[1] 杨立新[1] 王良华[1] 叶贤林[1] 茹丽梅 孟庆云 

机构地区：[1]深圳市血液中心 [2]吉林省通化市妇幼保健院 [3]长春市妇产科医院

出　　处：《国际医药卫生导报》2005年第11期100-101,共2页International Medicine and Health Guidance News

摘　　要：目的对酶联检测结果阴性NAT筛查为HBVDNA阳性标本8份中的6份进行HBVDNA定量追踪分析,以期发现血清转换期标本。方法采用国产及进口试剂同时检测乙肝表面抗原(HBsAg),丙型肝炎抗体(抗-HCV),爱滋病抗体(抗-HIV),梅毒螺旋体抗体(抗-TP),丙氨酸氨基转移酶(ALT),对各项结果均为阴性者进行核酸检测(NAT)筛查,对HBVDNA阳性献血员进行核酸定量追踪。结果从16512人份标本中筛出8份HBVDNA阳性,其中7例HBVDNA定量检测均能检测出HBVDNA的拷贝数,1例因HBVDNA含量过低不能检出拷贝数;随后追踪标本中HBVDNA含量均无显著性变化,未发现血清转换期献血员。结论所用酶联检测试剂及NAT筛查试剂均有较高灵敏度,核酸定量检测试剂定量较准确,HBVDNA定性检测结果为阳性并不一定说明体内有较高含量的HBVDNA拷贝数;应加大核酸筛查力度,才能发现血清转换期标本,从而为卫生部门制定相关政策提供科学依据。Objects We investigated 8 blood donors who were HBsAg, Anti-HCV, anti-HIV and anti- TP negative, ALT normal, nucleic acid testing(NAT) HBVDNA positive to find HBV serum conversions. Method We found 8 HBVDNA positive blood donors among 16512 blood donors and quantitated seven of them, one cannot be quantitated because of low level of HBVDNA. The HBVDNA level of the followed specimens did not vary significantly and found no serum conversions. Conclusion The reagents for EIA and NAT had relatively high sensitivity and quantitation reagents were relatively precise. We should detect more specimens in order to find serum conversions so that we could offer scientific data for the health administration.

关 键 词：追踪分析 HBSAG阴性 献血员 丙氨酸氨基转移酶(ALT) HBVDNA阳性 HBVDNA含量 HBVDNA定量检测 HBVDNA定性 梅毒螺旋体抗体 深圳 血清转换 乙肝表面抗原 丙型肝炎抗体 酶联检测试剂 DNA拷贝数 检测结果 核酸定量 阳性标本 

分 类 号：R446.6[医药卫生—诊断学] R446.5[医药卫生—临床医学]