伤寒杆菌抗原酶免法、LPS-PHA在伤寒早期诊断中的应用  

Apply of Immunoassay of Typhoid Bacillus Antigen and LPS-PHA on the Early Diagnosis of Typhoid

作　　者：王伟[1] 马华[1] 李杰[2] 李曼[3] 

机构地区：[1]安徽省蚌埠医学院医学检验系免疫学教研室,233003 [2]蚌埠医学院附属医院检验科 [3]山东省临沂市中医医院检验科

出　　处：《实用全科医学》2005年第4期367-367,共1页Applied Journal Of General Practice

摘　　要：目的探讨伤寒杆菌抗原酶免法(ELISA)、脂多糖-被动血凝试验(LPS-PHA)在伤寒早期诊断中的应用价值。方法选择224例门诊发热疑似伤寒患者,于发病第3、4、7d,分别用ELISA法、LPS-PHA、血培养、肥达氏反应检测伤寒杆菌。结果伤寒杆菌抗原酶免法(ELISA)、LPS-PHA在第7d的检出率分别为85.4%和80.5%,显著高于血培养和肥达氏反应(P<0.05)。结论伤寒杆菌抗原酶免法(ELISA)、LPS-PHA在伤寒早期诊断中具有较高的应用价值,但二者都有一定的假阳性和假阴性,联合应用对伤寒的早期辅助诊断更有价值。Objective To explore the value of immunoassay of typhoid antigen(ELISA) and lipopolysaccharide-passive hemagglutination assay (LPS-PHA) on the early diagnosis of typhoid. Methods 224 suspicious outpatients with typhoid were tested by ELISA, LPS-PHA, blood culture, Widal test on 3rd day, 4th day, 7th day. Results The positive rate of immunoassay of typhoid (ELISA) and LPS-PHA in typhoid individuals were 85.4%, 80.5% on 7th day, both of them were much higher than those of blood culture and Widal test (P<0.05). Conclusions ELISA and LPS-PHA have the good value on early diagnosis of typhoid, but both of them have false positive and false negative. Associated application of ELISA and LPS-PHA for early diagnosis of typhoid has a higher applied value.

关 键 词：LPS-PHA 伤寒杆菌抗原 早期诊断 酶免法 肥达氏反应 被动血凝试验 ELISA法 早期辅助诊断 应用价值 方法选择 脂多糖 血培养 检出率 假阴性 假阳性 

分 类 号：R516.304[医药卫生—内科学] R516.3[医药卫生—临床医学]