年龄因素对大鼠骨髓间充质干细胞数量及组织中BMP2含量的影响  

Influence of age on the amount of MSCs and values of BMP2 in serum and cortical bone in rats

作　　者：汤亭亭[1] 岳冰[1] 陆斌[1] 郁朝锋[1] 楼觉人[2] 戴尅戎[1] 

机构地区：[1]上海第二医科大学附属第九人民医院骨科,上海200011 [2]美国华盛顿大学医学院骨科实验室

出　　处：《中国骨质疏松杂志》2005年第2期156-158,163,共4页Chinese Journal of Osteoporosis

基　　金：上海市青年科技启明星(99QB14021);上海市科委重点项目(024119016)

摘　　要：目的观察增龄对大鼠骨髓间充质干细胞(mesenchymal stromal cells,MSCs)数量及组织中骨形态发生蛋白2(bone morphogenetic proteins 2,BMP2)含量的影响,分析MSCs数量和 BMP2含量变化与老年人骨量丢失及老年性骨质疏松症间的关系。方法取3种年龄层次的大鼠 (1月龄、9月龄、24月龄),抽取骨髓进行体外培养,计算纤维细胞集落形成单位(colony forming units dfibroblastic,CFU-F)的数量;应用ELISA技术定量检测大鼠外周血以及股骨皮质骨中 BMP2的含量。结果 MSCs数量随增龄显著减少。1月龄组皮质骨中以及血清中BMP2含量均明显高于其它两组,9月龄与24月龄大鼠间没有显著差异。结论骨髓中MSCs的数量随增龄而减少。外周血和皮质骨中BMP2的含量随增龄而减少。骨髓MSCs数量的减少及组织BMP2含量的降低可能是老年骨量丢失与老年性骨质疏松发病的重要原因。Objective To observe the differences of the number of MSCs and BMP2 value in Wistar rats of different ages (4-week-old, 9-month-old, and 24-month-old) and to analyze the relationship between those changes and bone loss and osteoporosis in elderly. Methods Primary MSCs were cultured in 10 nM dexam-ethasone media. On day 10, the number of CFU-F (fibroblast colony forming unit, CFU-F) and the ratio of ALP+ /CFU-F were counted, the contents of BMP2 were assayed by ELISA . Results For a given number of marrow cells there are fewer stem cells with osteogenic potential in older animals than there are in younger animals. The number of CFU-F of the 4-week-old, 9-month-old, and 24-month-old groups were 33. 67±3. 92, 24. 83 + 3. 54 and 18. 17±3. 97, respectively (P<0. 05), but colonies in these three groups all became alkaline phosphatase positive at the same rate (100%) . The contents of BMP2, both in serum and cortical bone, were highest in the 4-week-old group and lower in the other two groups. (serum : 122pg/mg vs 96, 2 pg/mg and 92. 7 pg/mg : cortical bone: 180pg/mg vs 122pg/mg and 106 pg/mg) Conclusion The defects in the number of MSCs and the content of BMP2 in tissues may underline the age-related decrease in bone formation.

关 键 词：BMP2 干细胞数量 组织 大鼠 年龄因素 骨髓间充质干细胞 老年性骨质疏松症 骨形态发生蛋白 MSCs 集落形成单位 骨量丢失 cells ELISA 皮质骨 含量变化 年龄层次 体外培养 纤维细胞 鼠外周血 定量检测 4月龄 增龄 老年人 

分 类 号：R657.3[医药卫生—外科学] R331.2[医药卫生—临床医学]