罗汉果多糖的分离纯化及分析  

Study on the Extracting Technology of Polysaccharides from Siraitia Grosvenorii

作　　者：李俊[1] 陈海燕[1] 邓胜平 苏小建[1] 义祥辉[1] 

机构地区：[1]广西师范大学资源与环境学系,广西桂林541004 [2]桂林斯达药业公司,广西桂林541004

出　　处：《化学世界》2005年第5期277-280,共4页Chemical World

基　　金：广西省高校基金资助项目(桂教科研2002第316号)

摘　　要：采用热水法提取罗汉果多糖,用Sevag法去除蛋白质,通过用DEAE 纤维素柱和SephadexG 200柱的分离纯化得到2个多糖组分。经测定,其相对分子质量分别为Mr1=430000,Mr2=650000。组分1的单糖组成:葡萄糖、半乳糖、木糖、阿拉伯糖、鼠李糖和葡萄糖醛酸;组分2的单糖组成;鼠李糖、葡萄糖醛酸。The extraction of polysaccharides from siraitia grosvenorii (SGPS) was studied. The crude polysaccharides was purified by chromatographic columns of DEAE-cellulose and Sephadex G-200 to get SGPS1 and SGPS2, respectively. According to TLC, SGPS1 was composed of D-glucose, D-galactose, D-xylose, L-arabiose, L-rhamnose and glycuronic acid. SGPS2 was composed of L-rhamnose and glycuronic acid. Their homogenity and molecular mass were measured by HPLC. Relative molecular mass of SGPS1 and SGPS2 were 430000 and 650000, respectively.

关 键 词：罗汉果 多糖 分离纯化 

分 类 号：TQ225.1[化学工程—有机化工]