RP-HPLC法分析优选黄连提取方法  

Analysis and select extraction method of Coptis Chinensis by RP-HPLC

作　　者：张学顺[1] 赵晓义[1] 郭仲平[1] 

机构地区：[1]山东中医药大学附属医院,济南250011

出　　处：《齐鲁药事》2005年第5期281-282,共2页qilu pharmaceutical affairs

基　　金：山东中医药大学附属医院科技发展项目资助

摘　　要：目的比较黄连不同提取方法所得提取液的整体成分及小檗碱的含量,确定黄连药材的最佳提取条件。方法采用反相色谱柱,以乙腈-磷酸二氢钾溶液(0.05mol·L-1)(47∶53)1000ml中加入SDS1.7g为流动相,流速1.0ml·min-1,检测波长为346nm。结果及结论不同提取方法所得提取液成分大体一致,75%乙醇索氏提取法所得小檗碱含量最高,95%乙醇索氏提取出的成分种类稍多一些。Objective To compare the whole components and Berberine in extraction fluid by different methods,and determine the best extracting conditions of Coptis Chinensis.Method RP-HPLC column was used.Acetonitrile-monopotassium phosphate(0.05mol·L -1 )(47∶53)1000ml added 1.7g SDS was as mobile phase.The flow rate was 1.0ml·min -1 and the detective wavelength was at 346nm.Results and conclusion The extraction fluid components by different methods were general consistent.The determination of Berberine in 75% ethanol Soxhlet was the most,and verity components extracted in 95% ethanol Soxhlet were a little more.

关 键 词：反相高效液相色谱法 黄连 提取 

分 类 号：TQ460.7[化学工程—制药化工]