降糖明目胶囊对糖尿病视网膜新生血管抑制作用的观察  

Inhibitory Effects of Neovasculariztion by Jiangtangmingmu Capsule on Mouse Diabetic Retinopathy

作　　者：王津津[1] 张伟[1] 卢清君[1] 李玮[1] 支楠[2] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心 [2]首都医科大学附属北京同仁医院中医科

出　　处：《首都医科大学学报》2005年第3期270-273,共4页Journal of Capital Medical University

摘　　要：目的观察降糖明目胶囊对STZ-糖尿病小鼠视网膜新生血管的抑制作用。方法用链脲佐菌素(STZ)建立糖尿病小鼠模型。实验小鼠随机分为正常对照组、STZ模型对照组、降糖明目胶囊治疗大、中、小剂量组(分别为临床用药的18、9、4.5倍),每天灌胃给药。在诱发糖尿病后第3、7、30天和第60天,测空腹血糖,并称体质量。在诱发后第80天,进行视网膜血管计数及病理观察。结果模型组小鼠体质量明显降低,空腹血糖明显升高,视网膜内血管计数增高,视网膜节细胞神经纤维层增厚,节细胞核排列紊乱;降糖明目胶囊大、中、小剂量治疗组可显著缓解体质量减轻症状,大、中剂量组还对小鼠空腹血糖的升高有明显的抑制作用,大、中、小剂量组可使视网膜内血管计数较STZ-糖尿病小鼠明显降低,视网膜节细胞神经纤维层改变不明显。结论降糖明目胶囊对STZ诱发糖尿病小鼠的视网膜新生血管增生有抑制作用。Objective To observe the inhibition of neovascularization by jiangtangmingmu capsule on mouse diabetic retinopathy. Methods Male C57BL/B mice was injected with STZ to induce the diabetic mouse model, which was accepted to be nice model to study the diabetic retinopathy. Three groups were treated with serious doses of jiangtangmingmu capsule, the doses were 18, 9, 4.5 folds of clinical application respectively. Two positive treatment control groups were subjected with two other clinic approved medicine. Medicines were applied intragastric feeding daily. Serum glucose and body weight were observed periodically. After 80 d treatment, retina sections were stained by HE method and neovascular were counted under biological microscope. Results Diabetic mice induced by STZ were observed as raised serum glucose, and body weight lost significantly. Retina sections with HE stained were counted as increased neovascular numbers. All treated groups, including the serious doses of jiangtangmingmu capsule, two approved medicines, were observed as serum glucose decreasing and body weight increasing compared to the untreated diabetic mice. And the neovascular counting was decreased after the medicine treatment. Conclusion Jiangtangmingmu capsule can inhibit retinal neovascularization of diabetic mice induced by STZ.

关 键 词：降糖明目胶囊 视网膜新生血管 抑制作用 链脲佐菌素(STZ) 视网膜节细胞 糖尿病小鼠 神经纤维层 空腹血糖 血管计数 视网膜内 正常对照组 体质量减轻 小剂量 小鼠模型 实验小鼠 临床用药 灌胃给药 病理观察 血管增生 诱发 

分 类 号：R259.872[医药卫生—中西医结合] R774.1[医药卫生—中医内科学]