RGDS肽对离体大鼠血小板聚集和ERK1/2磷酸化的影响  

Effects of RGDS Peptide on Aggregation and Phosphorylation of ERK1/2 in Rat Platelets in Vitro

作　　者：张立克[1] 刘玉玲[1] 芦玲巧[1] 姚兴海[1] 

机构地区：[1]首都医科大学病理生理学教研室

出　　处：《首都医科大学学报》2005年第3期291-292,共2页Journal of Capital Medical University

基　　金：北京市教委科技发展计划(99KJ15)资助项目

摘　　要：目的观察外源性精-甘-天-丝冬氨酸(RGDS)肽对离体大鼠血小板聚集及细胞外信号调节激酶1/2(ERK1/2)磷酸化的影响,探讨RGDS肽对血小板聚集的作用与ERK1/2磷酸化表达改变的关系。方法用比浊法测定血小板最大聚集率;Western-blot测定血小板ERK1/2磷酸化表达。结果RGDS肽抑制血小板聚集后伴随ERK1/2磷酸化表达减少。结论ERK1/2通路参与血小板聚集;抑制ERK1/2磷酸化表达可能是外源性RGDS肽抗血栓的机制之一。Objective To obsere influence of RGDS peptide on rat platelet aggregation rate and the ERK1/2 phosphorylation induced by different agonists in vivo and study the relationship between the change of ERK1/2 phosphorylation and the change of platelets aggregation with RGDS peptide. Methods Maximal aggregation rate(MAR) was measured with nephelometry; ERK1/2 phosphorylation was detected by Western blot. Results The MAR and phosphorylation of ERK1/2 were inhibited by RGDS peptide. Conclusion The phosphorylation of ERK1/2 was one of the cellular signal transduction mechanisms of the platelets aggregation; and the inhibition of ERK1/2 phosphorylation of may be one of the mechanisms that RGDS peptide antagonize thrombus.

关 键 词：ERK1/2 血小板聚集 RGDS肽 离体大鼠 磷酸化 细胞外信号调节激酶1 血小板最大聚集率 Western blot 表达减少 外源性 比浊法 抗血栓 测定 抑制 

分 类 号：R392[医药卫生—免疫学] R285.5[医药卫生—基础医学]